About

MiXCR is a software platform for immune profiling with Next-Generation Sequencing (NGS) data. It is used by majority of Big Pharma in the R&D for vaccine development, antibody discovery and cancer immunotherapy research.

MiXCR provides a seamless analytical pipeline starting from raw sequencing data to biological insights. It works with any wet lab protocol and data types. It extracts repertores from target amplicon sequencing protocols with and without molecular barcodes, shotgun and RNA-Seq libraries, various single cell technologies and is flexible to be tuned for any custom protocol.

Out of the box, it provides dedicated analytical pipelines for all kits from popular commercial vendors, standard sequencing data types and widely adopted public protocols. The whole analytical pipeline can be conducted as easy as one line. Few examples:

mixcr analyze 10x-vdj-bcr \
      --species hsa \
      in_R1.fq.gz \
      in_R2.fq.gz \
      output

mixcr analyze takara-human-bcr-full-length \
      in_R1.fq.gz \
      in_R2.fq.gz \
      output

mixcr analyze rnaseq-cdr3 \
      --species hsa \
      in_R1.fq.gz \
      in_R2.fq.gz \
      output

A full list of available presets can be found here.

The downstream analysis provided by MiXCR includes:

• data normalization
• inference of novel allelic variants
• reconstruction of B cell lineages
• various types of repertoire profiles
• powerful functionality to build repertoires overlap

Check out more in our reference documentation and tutorial guides.