Multiplex BCR cDNA libraries

For this tutorial we will use the data published in the following article:

Aizik L, Dror Y, Taussig D, Barzel A, Carmi Y, Wine Y. Antibody Repertoire Analysis of Tumor-Infiltrating B Cells Reveals Distinct Signatures and Distributions Across Tissues. Front Immunol. 2021 Jul 19;12:705381. doi: 10.3389/fimmu.2021.705381. PMID: 34349765; PMCID: PMC8327180.

Experiment setting

BALB/C mice were injected subcutaneously into mammary fat-pad number five with 2×105 4T1 cells suspended in 30 µl of DMEM (Gibco, Thermo Fisher Scientific). The induced tumors were monitored by measuring their size twice a week using calipers. After 23 days, mice were sacrificed, and B cells were isolated from four tissue types, namely, bone marrow, blood, DLNs, and tumors. For the control mice, three tissue types were collected - bone marrow, blood, and lymph nodes. Lymphocytes were isolated from every sample. For all tissues, cells were then incubated with a mixture of anti-IgG, anti-IgM, anti-CD138 magnetic beads. Later, total RNA was isolated following by first-strand cDNA synthesis using SuperScript™ III First-Strand Synthesis System with 200 ng RNA as the template and Oligo (dT) primers. After cDNA synthesis, PCR amplification of the variable heavy Ig genes was performed using a set of 19 forward primers with the gene-specific regions annealing to framework 1 of the VDJ-region and two reverse primers with the gene-specific region binding to the IgG and IgM constant regions. Recovered DNA products from the first PCR was applied to a second PCR amplification to attach Illumina adaptors to the amplified VH genes using the primer extension method. Technical replicates (two per sample) of BCR-Seq libraries were prepared based on cDNA from each mouse/tissue. cDNA was split, and library preparation was performed in parallel with different Illumina indices as described above. cDNA libraries were subjected to NGS on the MiSeq platform with the reagent kit V3 2 × 300 bp paired-end (Illumina).

Show primers

# PCR1 Forward primers
# Primers include step-out(glue) sequence and gene specific region

m-VH-glue-Fw1   CCCTCCTTTAATTCCCGAKGTRMAGCTTCAGGAGTC
m-VH-glue-Fw2   CCCTCCTTTAATTCCCGAGGTBCAGCTBCAGCAGTC
m-VH-glue-Fw3   CCCTCCTTTAATTCCCCAGGTGCAGCTGAAGSASTC
m-VH-glue-Fw4   CCCTCCTTTAATTCCCGAGGTCCARCTGCAACARTC
m-VH-glue-Fw5   CCCTCCTTTAATTCCCCAGGTYCAGCTBCAGCARTC
m-VH-glue-Fw6   CCCTCCTTTAATTCCCCAGGTYCARCTGCAGCAGTC
m-VH-glue-Fw7   CCCTCCTTTAATTCCCCAGGTCCACGTGAAGCAGTC
m-VH-glue-Fw8   CCCTCCTTTAATTCCCGAGGTGAASSTGGTGGAATC
m-VH-glue-Fw9   CCCTCCTTTAATTCCCGAVGTGAWGYTGGTGGAGTC
m-VH-glue-Fw10  CCCTCCTTTAATTCCCGAGGTGCAGSKGGTGGAGTC
m-VH-glue-Fw11  CCCTCCTTTAATTCCCGAKGTGCAMCTGGTGGAGTC
m-VH-glue-Fw12  CCCTCCTTTAATTCCCGAGGTGAAGCTGATGGARTC
m-VH-glue-Fw13  CCCTCCTTTAATTCCCGAGGTGCARCTTGTTGAGTC
m-VH-glue-Fw14  CCCTCCTTTAATTCCCGARGTRAAGCTTCTCGAGTC
m-VH-glue-Fw15  CCCTCCTTTAATTCCCGAAGTGAARSTTGAGGAGTC
m-VH-glue-Fw16  CCCTCCTTTAATTCCCCAGGTTACTCTRAAAGWGTSTG
m-VH-glue-Fw17  CCCTCCTTTAATTCCCCAGGTCCAACTVCAGCARCC
m-VH-glue-Fw18  CCCTCCTTTAATTCCCGATGTGAACTTGGAAGTGTC
m-VH-glue-Fw19  CCCTCCTTTAATTCCCGAGGTGAAGGTCATCGAGTC

# PCR1 Reverse primers
# Primers inglude step-out(glue) sequence and gene specific region

m-IgMC-BC-glue-REV      GAGGAGAGAGAGAGAGCGTGATCGAGGGGGAAGACATTTGGG
m-IgGall-BC-glue-REV    GAGGAGAGAGAGAGAGACATCGCCARKGGATAGACHGATGGG

# PCR2 Forward primer
# Primer ingludes Universal trueSeq Illumina adaptor and glue sequence from PCR1 forward primers

PE-IgALL-Univ-FW    AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNCCCTCCTTTAATTCCC

# PCR2 Reverse primer
# Primer ingludes Universal trueSeq Illumina adaptor and glue sequence from PCR1 reverse primers

YW23X_PE-Idx-REV    CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNGAGGAGAGAGAGAGAG

All data may be downloaded using the script bellow.

Use aria2c for efficient download of the full dataset with the proper filenames:

download.sh

mkdir -p raw
aria2c -c -s 16 -x 16 -k 1M -j 8 -i download-list.txt

download-list.txt

https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Blood_S2_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Blood_S2_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Blood_S6_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Blood_S6_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_BM_S4_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_BM_S4_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_BM_S8_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_BM_S8_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_DLN_S3_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_DLN_S3_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_DLN_S7_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_DLN_S7_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Tumor_S1_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Tumor_S1_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica1_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Tumor_S5_L001_R1_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M1_4T1_Tumor_S5_L001_R2_001_B3.1.fastq.gz
  out=raw/M1_4T1_replica2_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Blood_S2_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica1_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Blood_S2_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica1_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Blood_S6_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica2_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Blood_S6_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica2_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_BM_S4_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica1_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_BM_S4_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica1_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_BM_S8_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica2_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_BM_S8_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica2_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_DLN_S3_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica1_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_DLN_S3_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica1_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_DLN_S7_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica2_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_DLN_S7_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica2_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Tumor_S1_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica1_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Tumor_S1_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica1_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Tumor_S5_L001_R1_001.fastq.gz
  out=raw/M2_4T1_replica2_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M2_4T1_Tumor_S5_L001_R2_001.fastq.gz
  out=raw/M2_4T1_replica2_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Blood_S10_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica1_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Blood_S10_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica1_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Blood_S9_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica2_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Blood_S9_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica2_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_BM_S7_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica1_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_BM_S7_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica1_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_BM_S8_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica2_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_BM_S8_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica2_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_DLN_S11_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica1_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_DLN_S11_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica1_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_DLN_S12_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica2_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_DLN_S12_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica2_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Tumor_S13_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica1_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Tumor_S13_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica1_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Tumor_S14_L001_R1_001.fastq.gz
  out=raw/M3_4T1_replica2_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M3_4T1_Tumor_S14_L001_R2_001.fastq.gz
  out=raw/M3_4T1_replica2_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Blood_S2_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica1_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Blood_S2_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica1_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Blood_S7_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica2_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Blood_S7_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica2_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_BM_S10_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica1_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_BM_S10_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica1_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_BM_S5_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica2_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_BM_S5_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica2_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_DLN_S4_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica1_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_DLN_S4_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica1_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_DLN_S9_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica2_DLN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_DLN_S9_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica2_DLN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Tumor_S1_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica1_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Tumor_S1_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica1_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Tumor_S6_L001_R1_001.fastq.gz
  out=raw/M4_4T1_replica2_Tumor_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/M4_4T1_Tumor_S6_L001_R2_001.fastq.gz
  out=raw/M4_4T1_replica2_Tumor_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_Blood_S5_L001_R1_001.fastq.gz
  out=raw/N1_control_replica1_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_Blood_S5_L001_R2_001.fastq.gz
  out=raw/N1_control_replica1_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_Blood_S6_L001_R1_001.fastq.gz
  out=raw/N1_control_replica2_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_Blood_S6_L001_R2_001.fastq.gz
  out=raw/N1_control_replica2_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_BM_S1_L001_R1_001.fastq.gz
  out=raw/N1_control_replica1_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_BM_S1_L001_R2_001.fastq.gz
  out=raw/N1_control_replica1_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_BM_S2_L001_R1_001.fastq.gz
  out=raw/N1_control_replica2_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_BM_S2_L001_R2_001.fastq.gz
  out=raw/N1_control_replica2_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_LN_S3_L001_R1_001.fastq.gz
  out=raw/N1_control_replica1_LN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_LN_S3_L001_R2_001.fastq.gz
  out=raw/N1_control_replica1_LN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_LN_S4_L001_R1_001.fastq.gz
  out=raw/N1_control_replica2_LN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N1_control_LN_S4_L001_R2_001.fastq.gz
  out=raw/N1_control_replica2_LN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_Blood_S5_L001_R1_001.fastq.gz
  out=raw/N2_control_replica1_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_Blood_S5_L001_R2_001.fastq.gz
  out=raw/N2_control_replica1_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_Blood_S6_L001_R1_001.fastq.gz
  out=raw/N2_control_replica2_Blood_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_Blood_S6_L001_R2_001.fastq.gz
  out=raw/N2_control_replica2_Blood_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_BM_S1_L001_R1_001.fastq.gz
  out=raw/N2_control_replica1_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_BM_S1_L001_R2_001.fastq.gz
  out=raw/N2_control_replica1_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_BM_S2_L001_R1_001.fastq.gz
  out=raw/N2_control_replica2_BM_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_BM_S2_L001_R2_001.fastq.gz
  out=raw/N2_control_replica2_BM_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_LN_S3_L001_R1_001.fastq.gz
  out=raw/N2_control_replica1_LN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_LN_S3_L001_R2_001.fastq.gz
  out=raw/N2_control_replica1_LN_R2.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_LN_S4_L001_R1_001.fastq.gz
  out=raw/N2_control_replica2_LN_R1.fastq.gz
https://cdn.milaboratory.com/data/PRJNA699402/N2_control_LN_S4_L001_R2_001.fastq.gz
  out=raw/N2_control_replica2_LN_R2.fastq.gz

One command Solution

The easiest way to obtain clonotype tables for this type of data is to use a universal mixcr analyze command. This is a BCR data, so we will use a preset called generic-amplicon.

The exact command for a single sample you can see bellow:

mixcr analyze generic-amplicon \
    --species mmu \
    --rna \
    --rigid-left-alignment-boundary \
    --rigid-right-alignment-boundary \
    --tagPattern ^N{4}ccctcctttaattccc{21}(R1:*)\^N{4}gaggagagagagagagN{26}(R2:*) \
    --assemble-clonotypes-by {CDR1Begin:FR4End} \
    raw/M1_4T1_replica1_Blood_R1.fastq.gz \
    raw/M1_4T1_replica1_Blood_R2.fastq.gz \
    results/M1_4T1_replica1_Blood

The meaning of these options is the following.

--species: is set to mmu for Mus Musculus
--rna: corresponds to VTranscriptWithout5UTRWithP alignment feature for V-gene (see Gene features and anchor points for details)
--rigid-left-alignment-boundary: leads to a global alignment algorithms to align the left bound of V gene because primer sequences are trimmed with tagPattern. This options sets -OvParameters.parameters.floatingLeftBound=false for mixcr align step of the pipeline.
--rigid-right-alignment-boundary: sets -OjParameters.parameters.loatingRightBound=false -OcParameters.parameters.floatingRightBound=true for mixcr align step of the pipeline to ensure global alignment on the right bound of bot J and C gene. Global alignment should be used when primer sequences have been trimmed.
--tag-pattern ^N{4}ccctcctttaattcccN{22}(R2:*)\^N{4}gaggagagagagagagN{26}(R1:*): This tag-pattern trims all adapter and primer sequences from both reads.
--assemble-clonotypes-by {CDR1Begin:FR4End}: sets -OassemblingFeatures={CDR1Begin:FR4End} for mixcr assemble step. We extend the assembling feature to start from CDR1. That is because this is BCR data, where hypermutations occur throughout V gene, and we want to capture as much as we can.
raw/M1_4T1_replica1_Blood_R1.fastq.gz raw/M1_4T1_replica1_Blood_R2.fastq.gz results/M1_4T1_replica1_Blood: Finally, we provide the names of input files and an output prefix:

Running the command above will generate the following files:

> ls result/

# human-readable reports 
M1_4T1_replica1_Blood.align.report.txt
M1_4T1_replica1_Blood.align.report.json
M1_4T1_replica1_Blood.assemble.report.txt
M1_4T1_replica1_Blood.assemble.report.json

# raw alignments (highly compressed binary file)
M1_4T1_replica1_Blood.vdjca

# CDR3 clonotypes (highly compressed binary file)
M1_4T1_replica1_Blood.clns

# CDR3 clonotypes exported in tab-delimited txt
M1_4T1_replica1_Blood.clones.tsv

While .clns file holds all data and is used for downstream analysis using mixcr postanalisis, the output .tsv clonotype table will contain exhaustive information about each clonotype as well:

See first 100 records from FebControl1.clones.IGH.tsv clonotype table

cloneId	cloneCount	cloneFraction	targetSequences	targetQualities	allVHitsWithScore	allDHitsWithScore	allJHitsWithScore	allCHitsWithScore	allVAlignments	allDAlignments	allJAlignments	allCAlignments	nSeqFR1	minQualFR1	nSeqCDR1	minQualCDR1	nSeqFR2	minQualFR2	nSeqCDR2	minQualCDR2	nSeqFR3	minQualFR3	nSeqCDR3	minQualCDR3	nSeqFR4	minQualFR4	aaSeqFR1	aaSeqCDR1	aaSeqFR2	aaSeqCDR2	aaSeqFR3	aaSeqCDR3	aaSeqFR4	refPoints
0	17236	0.0390159	GGGTTCACCTTCACTGATTACTACATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTTATTAGAAACAAAGCTAATGGTTACACAACAGAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTACTGTGCAAGAGATGGTAACTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV7-3*00(2605.7)	IGHD2-8*00(30)	IGHJ2*00(490)	IGHM*00(64.8)	232\|460\|481\|0\|228\|SA234GSG282AST414CSG415AST457G\|2135.0	25\|31\|51\|228\|234\|\|30.0	19\|68\|68\|234\|283\|\|490.0	nan	nan	nan	GGGTTCACCTTCACTGATTACTAC	38	ATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTT	38	ATTAGAAACAAAGCTAATGGTTACACAACA	38	GAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTAC	38	TGTGCAAGAGATGGTAACTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFTDYY	MSWVRQPPGKALEWLGF	IRNKANGYTT	EYSASVKGRFTISRDNSQSILYLQMNTLRAEDSATYY	CARDGNYYFDYW	GQGTTLTVSS_	:::::0:24:75:105:216:-1:228:228:-8:-3:234:234:1:252:283::
1	12388	0.0280418	GGCTTCAACATTAAAGACACCTATATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGGATTGATCCTGCGAATGGTAATACTAAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTACTGTGCTAACTGGGACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV14-3*00(2523.9)	IGHD4-1*00(30)	IGHJ1*00(481)	IGHM*00(64.8)	232\|449\|471\|0\|217\|SA247GSC338AST369ASA433G\|2054.0	14\|20\|33\|217\|223\|\|30.0	22\|73\|73\|223\|274\|SA45G\|481.0	nan	nan	nan	GGCTTCAACATTAAAGACACCTAT	38	ATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGG	38	ATTGATCCTGCGAATGGTAATACT	38	AAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTAC	38	TGTGCTAACTGGGACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFNIKDTY	MHWVKQRPEQGLEWIGR	IDPANGNT	KYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYY	CANWDWYFDVW	GAGTTVTVSS_	:::::0:24:75:99:210:-2:217:217:-3:-2:223:223:-2:243:274::
2	6655	0.0150644	GGATTCACTTTCAGTAGCTATGGCATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGACATTACTACGGTAGTAGCTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2714.4)	IGHD1-1*00(85)	IGHJ2*00(490)	IGHM*00(64.8)	232\|451\|471\|0\|219\|\|2190.0	26\|43\|69\|220\|237\|\|85.0	19\|68\|68\|237\|286\|\|490.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGGC	38	ATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGACATTACTACGGTAGTAGCTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYG	MSWVRQTPDKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARHYYGSSYYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:220:-3:-3:237:237:1:255:286::
3	4321	0.00978113	GGGTTCACCTTCACTGATTACTACATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTTATTAGAAACAAAGCTAATGGTTACACAACAGAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTACTGTGCAAGAGATGGTAACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV7-3*00(2605.3)	nan	IGHJ2*00(480)	IGHM*00(64.9)	232\|460\|481\|0\|228\|SA234GSG282AST414CSG415AST457G\|2135.0	nan	20\|68\|68\|232\|280\|\|480.0	nan	nan	nan	GGGTTCACCTTCACTGATTACTAC	38	ATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTT	38	ATTAGAAACAAAGCTAATGGTTACACAACA	38	GAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTAC	38	TGTGCAAGAGATGGTAACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFTDYY	MSWVRQPPGKALEWLGF	IRNKANGYTT	EYSASVKGRFTISRDNSQSILYLQMNTLRAEDSATYY	CARDGNYFDYW	GQGTTLTVSS_	:::::0:24:75:105:216:-1:228:::::232:0:249:280::
4	3599	0.00814679	GGATTCACTTTCAGTAGCTTTGGAATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATACATTAGTAGTGGCAGTAGTACCATCTACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTACTGTGCAAGAAATGGGGATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-17*00(2477.8)	nan	IGHJ4*00(480)	IGHG2C*00(62.6)	232\|450\|471\|0\|218\|SG247ASA248GSA251TST300CSG379CSG414A\|2006.0	nan	26\|74\|74\|226\|274\|\|480.0	nan	nan	nan	GGATTCACTTTCAGTAGCTTTGGA	38	ATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATAC	38	ATTAGTAGTGGCAGTAGTACCATC	38	TACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGAAATGGGGATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GFTFSSFG	MHWVRQAPEKGLEWVAY	ISSGSSTI	YYADTVKGRFTISRDNPKNTLFLQMTSLRSEDTAMYY	CARNGDAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:-1:218:::::226:-6:243:274::
5	2414	0.00546439	GGCTACACATTTACTGACCATATTATGAATTGGGTAAAAAAGAGGCCTGGACAGGGCCTTGAGTGGATTGGAAGGATTTATCCAGTAAGTGGTGAAACTAACTACAATCAAAAGTTCATGGGCAAGGCCACATTCTCTGTAGACCGGTCCTCCAGCACAGTGTACATGGTGTTGAACAGCCTGACATCTGAGGACCCTGCTGTCTATTACTGTGGAACTGGGACGTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-11*00(2697.1)	IGHD4-1*00(40)	IGHJ2*00(460)	IGHM*00(64.9)	232\|449\|471\|0\|217\|\|2170.0	15\|23\|33\|217\|225\|\|40.0	22\|68\|68\|225\|271\|\|460.0	nan	nan	nan	GGCTACACATTTACTGACCATATT	38	ATGAATTGGGTAAAAAAGAGGCCTGGACAGGGCCTTGAGTGGATTGGAAGG	38	ATTTATCCAGTAAGTGGTGAAACT	38	AACTACAATCAAAAGTTCATGGGCAAGGCCACATTCTCTGTAGACCGGTCCTCCAGCACAGTGTACATGGTGTTGAACAGCCTGACATCTGAGGACCCTGCTGTCTATTAC	38	TGTGGAACTGGGACGTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDHI	MNWVKKRPGQGLEWIGR	IYPVSGET	NYNQKFMGKATFSVDRSSSTVYMVLNSLTSEDPAVYY	CGTGTYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:-2:217:217:-4:1:225:225:-2:240:271::
6	2409	0.00545308	GGGTTCACCTTCACTGATTACTACATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTTATTAGAAACAAAGCTAATGGTTACACAACAGAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTACTGTGCAAGAGATGGGACGTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV7-3*00(2605.2)	IGHD4-1*00(30)	IGHJ2*00(460)	IGHM*00(64.8)	232\|460\|481\|0\|228\|SA234GSG282AST414CSG415AST457G\|2135.0	17\|23\|33\|228\|234\|\|30.0	22\|68\|68\|234\|280\|\|460.0	nan	nan	nan	GGGTTCACCTTCACTGATTACTAC	38	ATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTT	38	ATTAGAAACAAAGCTAATGGTTACACAACA	38	GAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTAC	38	TGTGCAAGAGATGGGACGTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFTDYY	MSWVRQPPGKALEWLGF	IRNKANGYTT	EYSASVKGRFTISRDNSQSILYLQMNTLRAEDSATYY	CARDGTYFDYW	GQGTTLTVSS_	:::::0:24:75:105:216:-1:228:228:-6:1:234:234:-2:249:280::
7	2378	0.0053829	GGATACACATTCACTAGCTATGTTATGCACTGGGTGAAGCAGAAGCCTGGGCAGGGCCTTGAGTGGATTGGATATATTAATCCTTACAATGATGGTACTAAGTACAATGAGAAGTTCAAAGGCAAGGCCACACTGACTTCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCAGCAGCCTGACCTCTGAGGACTCTGCGGTCTATTACTGTGCAACTGGGTATTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-83*00(1915.8)	IGHD4-1*00(25)	IGHJ4*00(550)	IGHM*00(64.8)	182\|399\|421\|0\|217\|SG197ASA198GSC202TST203GSA204TSC205TSA233CSG254TSG256TST260ASG266TSG267ASA268CSG270ASG273ASA275GSA276GST281ASC283GSG301ASG320TSC327ASC350GSA367CSA382GST390A\|1416.0	15\|20\|33\|217\|222\|\|25.0	19\|74\|74\|222\|277\|\|550.0	nan	nan	nan	GGATACACATTCACTAGCTATGTT	38	ATGCACTGGGTGAAGCAGAAGCCTGGGCAGGGCCTTGAGTGGATTGGATAT	38	ATTAATCCTTACAATGATGGTACT	38	AAGTACAATGAGAAGTTCAAAGGCAAGGCCACACTGACTTCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCAGCAGCCTGACCTCTGAGGACTCTGCGGTCTATTAC	38	TGTGCAACTGGGTATTACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTSYV	MHWVKQKPGQGLEWIGY	INPYNDGT	KYNEKFKGKATLTSDKSSSTAYMELSSLTSEDSAVYY	CATGYYYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:-2:217:217:-4:-2:222:222:1:246:277::
8	2351	0.00532179	GGATTCACTTTCAGTAGCTATGCCATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACGGCCATGTATTACTGTGCAAGACATTACTACGGTAGTAGCTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-600(2543.7),IGHV5-900(2541.9)	IGHD1-1*00(85)	IGHJ2*00(490)	IGHM*00(64.9)	232\|451\|471\|0\|219\|SG254CSA279GSC282GSA416GSA429G\|2045.0;232\|451\|471\|0\|219\|SA253GSG313ASG322ASA325TST354GST433A\|2016.0	26\|43\|69\|220\|237\|\|85.0	19\|68\|68\|237\|286\|\|490.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGCC	38	ATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGACATTACTACGGTAGTAGCTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYA	MSWVRQTPEKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLRSEDTAMYY	CARHYYGSSYYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:220:-3:-3:237:237:1:255:286::
9	2128	0.004817	GGATTCACTTTCAGTAGCTATGCCATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGTCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACGGCCATGTATTACTGTGCAAGACATTACTACGGTAGTAGCTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-900(2568.8),IGHV5-600(2513.8)	IGHD1-1*00(85)	IGHJ2*00(490)	IGHM*00(64.7)	232\|451\|471\|0\|219\|SA253GSG313ASG322ASA325TST433A\|2045.0;232\|451\|471\|0\|219\|SG254CSA279GSC282GSG354TSA416GSA429G\|2016.0	26\|43\|69\|220\|237\|\|85.0	19\|68\|68\|237\|286\|\|490.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGCC	38	ATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGTCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGACATTACTACGGTAGTAGCTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYA	MSWVRQTPEKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLRSEDTAMYY	CARHYYGSSYYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:220:-3:-3:237:237:1:255:286::
10	1991	0.00450688	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTATGGTGATGCTAGCTACAACCAGAAGTTCAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGACTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGACCCTATGATTACGACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-1900(1634.1),IGHV1-2200(1601.9)	IGHD2-4*00(55)	IGHJ4*00(510)	IGHG2C*00(38.7)	232\|451\|471\|0\|219\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSC321TSG326ASA328GST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC426TSA432CSG433A\|1378.0;232\|451\|471\|0\|219\|SA234CSC249TSC252TSA253GSA254CSC255TSC276TSG281CSC288TST291AST304GSA305TSA311GSC312TSC313ASA316TSA319TSG326ASA328GST364ASA373GSG378ASC396TSG402ASC405TSC406GSG407CSC411ASG420TSA432CSG433A\|1291.0	21\|32\|51\|221\|232\|\|55.0	23\|74\|74\|232\|283\|\|510.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTATGGTGATGCT	38	AGCTACAACCAGAAGTTCAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGACTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGACCCTATGATTACGACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYYGDA	SYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARPYDYDYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:0:219:221:-4:-2:232:232:-3:252:283::
11	1819	0.00411754	GGGTTTTCATTAACCAGCTATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTAATATGGGCTGGTGGAAGCACAAATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTACTGTGCCAGAACTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-9*00(2581)	nan	IGHJ2*00(410)	IGHM*00(64.7)	232\|446\|469\|0\|214\|SG259CSG314CSA339G\|2053.0	nan	27\|68\|68\|218\|259\|\|410.0	nan	nan	nan	GGGTTTTCATTAACCAGCTATGGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTA	38	ATATGGGCTGGTGGAAGCACA	38	AATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGAACTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQPPGKGLEWLGV	IWAGGST	NYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CARTDYW	GQGTTLTVSS_	:::::0:24:75:96:207:-3:214:::::218:-7:228:259::
12	1793	0.00405868	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACAATGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATACTATGGTAACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1480.1)	IGHD2-800(45),IGHD2-700(40)	IGHJ2*00(480)	IGHM*00(64.8)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1301.0	20\|29\|51\|220\|229\|\|45.0;20\|28\|51\|220\|228\|\|40.0	20\|68\|68\|229\|277\|\|480.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACAATGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATACTATGGTAACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYNGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARYYGNYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:220:-3:-5:229:229:0:246:277::
13	1744	0.00394776	GGATACACATTCACTGACTATGTTATAAGCTGGGTGAAGCAGAGAACTGGACAGGGCCTTGAGTGGATTGGAGAGATTTATCCTGGAAGTGGTAGTACTTACTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAACACAGCCTACATGCAGCTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTTCTGTGCAAGATGGGAATTACGAAGGGGCGATTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-7700(2286.1),IGHV1-8100(2235)	IGHD2-400(35),IGHD2-700(30)	IGHJ4*00(540)	IGHG2C*00(55.1)	232\|452\|471\|0\|220\|SC234ASC240ASC252TST253GSA254TSA260GSG276ASC277ASA304GSG310TSG311ASG386ASA432G\|1823.0;232\|452\|471\|0\|220\|SC234ASC240ASA246TSA247GSG248ASG254TSA316GSA326GSG386ASG393CSG400CSC406A\|1852.0	26\|33\|51\|224\|231\|\|35.0;27\|33\|51\|225\|231\|\|30.0	20\|74\|74\|238\|292\|\|540.0	nan	nan	nan	GGATACACATTCACTGACTATGTT	38	ATAAGCTGGGTGAAGCAGAGAACTGGACAGGGCCTTGAGTGGATTGGAGAG	38	ATTTATCCTGGAAGTGGTAGTACT	38	TACTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAACACAGCCTACATGCAGCTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTTC	38	TGTGCAAGATGGGAATTACGAAGGGGCGATTACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYV	ISWVKQRTGQGLEWIGE	IYPGSGST	YYNEKFKGKATLTADKSSNTAYMQLSSLTSEDSAVYF	CARWELRRGDYYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:1:220:224:-9:-1:231:238:0:261:292::
14	1674	0.00378931	GGCTACACATTCAGTAGCTACTGGATAGAGTGGGTAAAGCAGAGGCCTGGACATGGCCTTGAGTGGATTGGAGAGATTTTACCTGGAAGTGGTAGTACTAACTACAATGAGAAGTTCAAGGGCAAGGCCACATTCACTGCAGATACATCCTCCAACACAGCCTACATGCAACTCAGCAGCCTGACATCTGAGGACTCTGCCGTCTATTACTGTGCCTCGACAGCTCGGGCTACGAGAGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-9*00(2502.8)	IGHD3-100(75),IGHD3-200(67)	IGHJ4*00(460)	IGHG2C*00(61.6)	232\|447\|471\|0\|215\|SC245GSG247ASA418TSA433G\|2034.0	20\|35\|51\|219\|234\|\|75.0;14\|33\|48\|215\|234\|SA16CSA25G\|67.0	28\|74\|74\|237\|283\|\|460.0	nan	nan	nan	GGCTACACATTCAGTAGCTACTGG	38	ATAGAGTGGGTAAAGCAGAGGCCTGGACATGGCCTTGAGTGGATTGGAGAG	38	ATTTTACCTGGAAGTGGTAGTACT	38	AACTACAATGAGAAGTTCAAGGGCAAGGCCACATTCACTGCAGATACATCCTCCAACACAGCCTACATGCAACTCAGCAGCCTGACATCTGAGGACTCTGCCGTCTATTAC	38	TGTGCCTCGACAGCTCGGGCTACGAGAGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFSSYW	IEWVKQRPGHGLEWIGE	ILPGSGST	NYNEKFKGKATFTADTSSNTAYMQLSSLTSEDSAVYY	CASTARATRAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:-4:215:219:-3:1:234:237:-8:252:283::
15	1615	0.00365576	GGCTACACCTTCACTACCTATCCAATAGAGTGGATGAAGCAGAATCATGGGAAGAGCCTAGAGTGGATTGGAAATTTTCATCCTTACAATGATGATACTAAGTACAATGAAAAATTCAAGGGCAAGGCCAAATTGACTGTAGAAAAATCCTCTAGCACAGTCTACTTGGAGCTCAGCCGATTAACATCTGATGACTCTGCTGTTTATTACTGTGCAAGGGGGGGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-47*00(2513.3)	nan	IGHJ3*00(450)	IGHM*00(64.9)	232\|451\|471\|0\|219\|ST255ASA282GSG345ASC362A\|2074.0	nan	23\|68\|68\|223\|268\|\|450.0	nan	nan	nan	GGCTACACCTTCACTACCTATCCA	38	ATAGAGTGGATGAAGCAGAATCATGGGAAGAGCCTAGAGTGGATTGGAAAT	38	TTTCATCCTTACAATGATGATACT	38	AAGTACAATGAAAAATTCAAGGGCAAGGCCAAATTGACTGTAGAAAAATCCTCTAGCACAGTCTACTTGGAGCTCAGCCGATTAACATCTGATGACTCTGCTGTTTATTAC	38	TGTGCAAGGGGGGGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYTFTTYP	IEWMKQNHGKSLEWIGN	FHPYNDDT	KYNEKFKGKAKLTVEKSSSTVYLELSRLTSDDSAVYY	CARGGFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:::::223:-3:237:268::
16	1570	0.00355389	GGATTCACCTTCAATACCTACGCCATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGTTTGGAATGGGTTGCTCGCATAAGAAGTAAAAGTAATAATTATGCAACATATTATGCCGATTCAGTGAAAGACAGGTTCACCATCTCCAGAGATGATTCACAAAGCATGCTCTATCTGCAAATGAACAACTTGAAAACTGAGGACACAGCCATGTATTACTGTGTGAGCGGGGGTAACTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV10-1*00(2679.8)	nan	IGHJ1*00(512)	IGHG2C*00(58.2)	232\|456\|477\|0\|224\|SG239CSA363GSG388C\|2153.0	nan	16\|73\|73\|229\|286\|SG19ASA45G\|512.0	nan	nan	nan	GGATTCACCTTCAATACCTACGCC	38	ATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGTTTGGAATGGGTTGCTCGC	38	ATAAGAAGTAAAAGTAATAATTATGCAACA	38	TATTATGCCGATTCAGTGAAAGACAGGTTCACCATCTCCAGAGATGATTCACAAAGCATGCTCTATCTGCAAATGAACAACTTGAAAACTGAGGACACAGCCATGTATTAC	38	TGTGTGAGCGGGGGTAACTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFTFNTYA	MNWVRQAPGKGLEWVAR	IRSKSNNYAT	YYADSVKDRFTISRDDSQSMLYLQMNNLKTEDTAMYY	CVSGGNYWYFDVW	GAGTTVTVSS_	:::::0:24:75:105:216:-1:224:::::229:4:255:286::
17	1544	0.00349504	GGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCGGAAAAGAGGCTGGAGTGGGTCGCAACCATTAGTGATGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACAACCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGTTATAGGTACCTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-400(2416.2),IGHV5-600(2387.6)	IGHD5-100(35),IGHD5-200(30),IGHD5-3*00(30)	IGHJ3*00(420)	IGHG2C*00(48.7)	232\|450\|471\|0\|218\|SA247GSG248ASG253TSC254ASC260ASA344GSA348GSC354GSC409ASA410G\|1890.0;232\|450\|471\|0\|218\|SA247GSG248ASG253TSG254ASC260ASA279GSC282ASA313GSG314ASC389A\|1890.0	1\|8\|30\|221\|228\|\|35.0;1\|7\|30\|221\|227\|\|30.0;1\|7\|30\|221\|227\|\|30.0	26\|68\|68\|229\|271\|\|420.0	nan	nan	nan	GGATTCACTTTCAGTGACTATTAC	38	ATGTATTGGGTTCGCCAGACTCCGGAAAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTGATGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACAACCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGTTATAGGTACCTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFSDYY	MYWVRQTPEKRLEWVAT	ISDGGSYT	YYPDSVKGRFTISRDNAKNNLYLQMSSLKSEDTAMYY	CASYRYLAYW	GQGTLVTVSA_	:::::0:24:75:99:210:-1:218:221:9:-12:228:229:-6:240:271::
18	1332	0.00301515	GAATACGAATTCCCTTCCCATGACATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCCATTAATAGTGATGGTGGTAGCACCTACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTACTGTGCAAGACACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-2*00(2737.2)	nan	IGHJ2*00(480)	IGHM*00(64.8)	232\|453\|473\|0\|221\|\|2210.0	nan	20\|68\|68\|220\|268\|\|480.0	nan	nan	nan	GAATACGAATTCCCTTCCCATGAC	38	ATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCC	38	ATTAATAGTGATGGTGGTAGCACC	38	TACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTAC	38	TGTGCAAGACACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	EYEFPSHD	MSWVRKTPEKRLELVAA	INSDGGST	YYPDTMERRFIISRDNTKKTLYLQMSSLRSEDTALYY	CARHYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:221:::::220:0:237:268::
19	1331	0.00301289	GGCTACACATTTACTGACTATGAAATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCTATTGATCCTGAAACTGGTGGTACTGCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTACTGTACAAGAAGGGACCATGGTAACTACGGCTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-15*00(2688.6)	IGHD2-8*00(61)	IGHJ2*00(380)	IGHG2C*00(64.2)	232\|451\|471\|0\|219\|ST362C\|2161.0	20\|35\|51\|223\|238\|ST22C\|61.0	30\|68\|68\|239\|277\|\|380.0	nan	nan	nan	GGCTACACATTTACTGACTATGAA	38	ATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCT	38	ATTGATCCTGAAACTGGTGGTACT	38	GCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTAC	38	TGTACAAGAAGGGACCATGGTAACTACGGCTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYE	MHWVKQTPVHGLEWIGA	IDPETGGT	AYNQKFKGKATLTADKSSSTAYMELRSLTSEDSAVYY	CTRRDHGNYGYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:223:-3:1:238:239:-10:246:277::
20	1297	0.00293592	GGCTACACATTTACTGACTATGAAATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCTATTGATCCTGAAACTGGTGGTACTGCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTACTGTACAAGACGCTACGGTAGTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-15*00(2688.3)	IGHD1-1*00(45)	IGHJ2*00(410)	IGHG2C*00(64.1)	232\|451\|471\|0\|219\|ST362C\|2161.0	30\|39\|69\|221\|230\|\|45.0	27\|68\|68\|230\|271\|\|410.0	nan	nan	nan	GGCTACACATTTACTGACTATGAA	38	ATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCT	38	ATTGATCCTGAAACTGGTGGTACT	38	GCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTAC	38	TGTACAAGACGCTACGGTAGTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYE	MHWVKQTPVHGLEWIGA	IDPETGGT	AYNQKFKGKATLTADKSSSTAYMELRSLTSEDSAVYY	CTRRYGSDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:221:-7:-7:230:230:-7:240:271::
21	1230	0.00278426	GGATTCACTTTCAGTAGCTATACCATGTCTTGGGTTCGCCAGACTCCAGAGAAGAGGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTACTATCCAGACACTGTAAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACGGCCATGTATTACTGTGCAAGACATTATTACTACGGTAGTAGCTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-12*00(2457.4)	IGHD1-1*00(95)	IGHJ2*00(490)	IGHM*00(64.9)	232\|451\|471\|0\|219\|SG247ASA248GST253ASA254CSA260CST333CSC409ASA429G\|1958.0	24\|43\|69\|221\|240\|\|95.0	19\|68\|68\|240\|289\|\|490.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATACC	38	ATGTCTTGGGTTCGCCAGACTCCAGAGAAGAGGCTGGAGTGGGTCGCATAC	38	ATTAGTAATGGTGGTGGTAGCACC	38	TACTATCCAGACACTGTAAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGACATTATTACTACGGTAGTAGCTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYT	MSWVRQTPEKRLEWVAY	ISNGGGST	YYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARHYYYGSSYYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:221-3:240:240:1:258:289::
22	1130	0.0025579	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATGGTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1425.2)	nan	IGHJ2*00(500)	IGHM*00(63.6)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1243.0	nan	18\|68\|68\|221\|271\|\|500.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATGGTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARWYYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:::::221:2:240:271::
23	1124	0.00254432	GGATTCACTTTCAGTGACTACGGAATGGCGTGGGTTCGACAGGCTCCAGGGAAGGGGCCTGAGTGGGTAGCATTCATTAGTAATTTGGCATATAGTATCTACTATGCAGACACTGTGACGGGCCGATTCACCATCTCTAGAGAGAATGCCAAGAACACCCTGTACCTGGAAATGAGCAGTCTGAGGTCTGAGGACACAGCCATGTACTACTGTGCAAGGGATTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-15*00(2590.3)	IGHD1-1*00(55)	IGHJ1*00(551)	IGHM*00(64.8)	232\|450\|471\|0\|218\|SA280GSG429AST438C\|2093.0	26\|37\|69\|220\|231\|\|55.0	15\|73\|73\|231\|289\|SA45G\|551.0	nan	nan	nan	GGATTCACTTTCAGTGACTACGGA	38	ATGGCGTGGGTTCGACAGGCTCCAGGGAAGGGGCCTGAGTGGGTAGCATTC	38	ATTAGTAATTTGGCATATAGTATC	38	TACTATGCAGACACTGTGACGGGCCGATTCACCATCTCTAGAGAGAATGCCAAGAACACCCTGTACCTGGAAATGAGCAGTCTGAGGTCTGAGGACACAGCCATGTACTAC	38	TGTGCAAGGGATTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFTFSDYG	MAWVRQAPGKGPEWVAF	ISNLAYSI	YYADTVTGRFTISRENAKNTLYLEMSSLRSEDTAMYY	CARDYYGSSYWYFDVW	GAGTTVTVSS_	:::::0:24:75:99:210:-1:218:220:-3:-9:231:231:5:258:289::
24	1074	0.00243113	GGCTTCAACATTAAAGACTACTATATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGATGGATTGATCCTGAGAATGGTAATACTATATATGACCCGAAGTTCCAGGGCAAGGCCAGTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTACTGTGCTAGAGGGGGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV14-200(2340.9),IGHV14-400(2324.4)	nan	IGHJ3*00(450)	IGHM*00(64.8)	232\|451\|471\|0\|219\|SA277CSA304TSG319ASG325ASA327TSA332TSC338ASA345GSC362G\|1929.0;232\|449\|471\|0\|217\|ST237CSG250TSG325ASG331ASA332TSC338AST340CSC362GSA445G\|1909.0	nan	23\|68\|68\|223\|268\|\|450.0	nan	nan	nan	GGCTTCAACATTAAAGACTACTAT	38	ATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGATGG	38	ATTGATCCTGAGAATGGTAATACT	38	ATATATGACCCGAAGTTCCAGGGCAAGGCCAGTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTAC	38	TGTGCTAGAGGGGGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFNIKDYY	MHWVKQRPEQGLEWIGW	IDPENGNT	IYDPKFQGKASITADTSSNTAYLQLSSLTSEDTAVYY	CARGGFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:::::223:-3:237:268::
25	1024	0.00231795	GGATACACATTCACTAGCTATGTTATGCACTGGGTGAAGCAGAAGCCTGGGCAGGGCCTTGAGTGGATTGGATATATTAATCCTTACAATGATGGTACTAAGTACAATGAGAAGTTCAAAGGCAAGGCCACACTGACTTCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCAGCAGCCTGACCTCTGAGGACTCTGCGGTCTATTACTGTGCAAGAGAGGGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-83*00(1934.7)	nan	IGHJ3*00(450)	IGHM*00(64.9)	182\|401\|421\|0\|219\|SG197ASA198GSC202TST203GSA204TSC205TSA233CSG254TSG256TST260ASG266TSG267ASA268CSG270ASG273ASA275GSA276GST281ASC283GSG301ASG320TSC327ASC350GSA367CSA382GST390A\|1436.0	nan	23\|68\|68\|223\|268\|\|450.0	nan	nan	nan	GGATACACATTCACTAGCTATGTT	38	ATGCACTGGGTGAAGCAGAAGCCTGGGCAGGGCCTTGAGTGGATTGGATAT	38	ATTAATCCTTACAATGATGGTACT	38	AAGTACAATGAGAAGTTCAAAGGCAAGGCCACACTGACTTCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCAGCAGCCTGACCTCTGAGGACTCTGCGGTCTATTAC	38	TGTGCAAGAGAGGGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYTFTSYV	MHWVKQKPGQGLEWIGY	INPYNDGT	KYNEKFKGKATLTSDKSSSTAYMELSSLTSEDSAVYY	CAREGFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:::::223:-3:237:268::
26	1014	0.00229532	GGCTACACATTTACTGACTATGAAATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCTATTGATCCTGAAACTGGTGGTACTGCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTACTGTACAAGATGGTTACTACCTTTCGACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-15*00(2697.9)	IGHD2-3*00(45)	IGHJ4*00(510)	IGHG2C*00(63.5)	232\|452\|471\|0\|220\|ST362C\|2171.0	25\|34\|51\|220\|229\|\|45.0	23\|74\|74\|235\|286\|\|510.0	nan	nan	nan	GGCTACACATTTACTGACTATGAA	38	ATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCT	38	ATTGATCCTGAAACTGGTGGTACT	38	GCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTAC	38	TGTACAAGATGGTTACTACCTTTCGACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYE	MHWVKQTPVHGLEWIGA	IDPETGGT	AYNQKFKGKATLTADKSSSTAYMELRSLTSEDSAVYY	CTRWLLPFDYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:1:220:220:-8:0:229:235:-3:255:286::
27	1001	0.00226589	GGCTACACATTTACTGACTATGAAATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCTATTGATCCTGAAACTGGTGGTACTGCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTACTGTACAAGACGGTTTCCTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-15*00(2687.8)	IGHD5-700(25),IGHD5-800(25),IGHD6-2*00(25)	IGHJ2*00(410)	IGHG2C*00(64.1)	232\|451\|471\|0\|219\|ST362C\|2161.0	57\|62\|87\|219\|224\|\|25.0;57\|62\|87\|219\|224\|\|25.0;57\|62\|87\|219\|224\|\|25.0	27\|68\|68\|227\|268\|\|410.0	nan	nan	nan	GGCTACACATTTACTGACTATGAA	38	ATGCACTGGGTGAAGCAGACACCTGTGCATGGCCTGGAATGGATTGGAGCT	38	ATTGATCCTGAAACTGGTGGTACT	38	GCCTACAATCAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCCGTCTATTAC	38	TGTACAAGACGGTTTCCTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYE	MHWVKQTPVHGLEWIGA	IDPETGGT	AYNQKFKGKATLTADKSSSTAYMELRSLTSEDSAVYY	CTRRFPDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:219:-28:4:224:227:-7:237:268::
28	998	0.0022591	GGTTACTCATTCACTGGCTACTACATGCACTGGGTGAAGCAGAGCCATGGAAAGAGCCTTGAGTGGATTGGACGTGTTAATCCTAACAATGGTGGTACTAGCTACAACCAGAAGTTCAAGGGCAAGGCCATATTAACTGTAGACAAGTCATCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCGGTCTATTACTGTGAAATCTACTATGGTAACTCCGGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-2600(2261.7),IGHV1-3400(2204.2)	IGHD2-8*00(75)	IGHJ3*00(451)	IGHM*00(64.7)	232\|449\|471\|0\|217\|SA234TSA238TSG240ASA248GSA259CSG304CSA305GSA307GSC362TSG366ASC381ASA432GSC446A\|1793.0;232\|449\|471\|0\|217\|SC234TSA238TSA248GST304CSA305GSA307GST310ASA329CSG331ASC362TSG366ASC381ASA432GSC446A\|1764.0	17\|32\|51\|217\|232\|\|75.0	20\|68\|68\|232\|280\|ST22G\|451.0	nan	nan	nan	GGTTACTCATTCACTGGCTACTAC	38	ATGCACTGGGTGAAGCAGAGCCATGGAAAGAGCCTTGAGTGGATTGGACGT	38	GTTAATCCTAACAATGGTGGTACT	38	AGCTACAACCAGAAGTTCAAGGGCAAGGCCATATTAACTGTAGACAAGTCATCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCGGTCTATTAC	38	TGTGAAATCTACTATGGTAACTCCGGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYSFTGYY	MHWVKQSHGKSLEWIGR	VNPNNGGT	SYNQKFKGKAILTVDKSSSTAYMELRSLTSEDSAVYY	CEIYYGNSGFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:-2:217:217:0:-2:232:232:0:249:280::
29	962	0.00217761	GGGTTCACCTTCACTGATTACTACATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTTATTAGAAACAAAGCTAATGGTTACACAACAGAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTACTGTGCAAGAGATGGTAACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV7-3*00(2605)	nan	IGHJ1*00(481)	IGHM*00(65)	232\|460\|481\|0\|228\|SA234GSG282AST414CSG415AST457G\|2135.0	nan	22\|73\|73\|232\|283\|SA45G\|481.0	nan	nan	nan	GGGTTCACCTTCACTGATTACTAC	38	ATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTT	38	ATTAGAAACAAAGCTAATGGTTACACAACA	38	GAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTAC	38	TGTGCAAGAGATGGTAACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFTFTDYY	MSWVRQPPGKALEWLGF	IRNKANGYTT	EYSASVKGRFTISRDNSQSILYLQMNTLRAEDSATYY	CARDGNWYFDVW	GAGTTVTVSS_	:::::0:24:75:105:216:-1:228:::::232:-2:252:283::
30	957	0.00216629	GGATTCACTTTCAGTAACTACTGGATGAACTGGGTCCGCCAGTCTCCAGAGAAGGGGCTTGAGTGGGTTGCTGAAATTAGATTGAAATCTAATAATTATGCAACACATTATGCGGAGTCTGTGAAAGGGAGGTTCACCATCTCAAGAGATGATTCCAAAAGTAGTGTCTACCTGCAAATGAACAACTTAAGAGCTGAAGACACTGGCATTTATTACTGTACCAGGAGCTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV6-3*00(2590.8)	nan	IGHJ3*00(430)	IGHM*00(64.9)	232\|453\|476\|0\|221\|SC304GSG322ASG423ASA438CSC450T\|2065.0	nan	25\|68\|68\|228\|271\|\|430.0	nan	nan	nan	GGATTCACTTTCAGTAACTACTGG	38	ATGAACTGGGTCCGCCAGTCTCCAGAGAAGGGGCTTGAGTGGGTTGCTGAA	38	ATTAGATTGAAATCTAATAATTATGCAACA	38	CATTATGCGGAGTCTGTGAAAGGGAGGTTCACCATCTCAAGAGATGATTCCAAAAGTAGTGTCTACCTGCAAATGAACAACTTAAGAGCTGAAGACACTGGCATTTATTAC	38	TGTACCAGGAGCTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFSNYW	MNWVRQSPEKGLEWVAE	IRLKSNNYAT	HYAESVKGRFTISRDDSKSSVYLQMNNLRAEDTGIYY	CTRSFAYW	GQGTLVTVSA_	:::::0:24:75:105:216:-3:221:::::228:-5:240:271::
31	917	0.00207575	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATACGGTAGTAGCTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1426.3)	IGHD1-1*00(50)	IGHJ2*00(470)	IGHM*00(64.9)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1243.0	32\|42\|69\|220\|230\|\|50.0	21\|68\|68\|230\|277\|\|470.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATACGGTAGTAGCTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARYGSSYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:220:-9:-4:230:230246:277::
32	912	0.00206443	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATACGGTAGTAGCTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1454.4)	IGHD1-1*00(55)	IGHJ1*00(431)	IGHM*00(64.8)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1243.0	32\|43\|69\|220\|231\|\|55.0	27\|73\|73\|231\|277\|SA45G\|431.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATACGGTAGTAGCTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARYGSSYFDVW	GAGTTVTVSS_	:::::0:24:75:99:210:1:220:220:-9:-3:231:231:-7:246:277::
33	894	0.00202368	GGATTCACTTTCAGTAACTACTGGATGAACTGGGTCCGCCAGTCTCCAGAGAAGGGGCTTGAGTGGGTTGCTGAAATTAGATTGAAATCTAATAATTATGCAACACATTATGCGGAGTCTGTGAAAGGGAGGTTCACCATCTCAAGAGATGATTCCAAAAGTAGTGTCTACCTGCAAATGAACAACTTAAGAGCTGAAGACACTGGCATTTATTACTGTACCAGGGCGGGAAACTCGGCCTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV6-3*00(2591.9)	nan	IGHJ3*00(502)	IGHM*00(64.7)	232\|453\|476\|0\|221\|SC304GSG322ASG423ASA438CSC450T\|2065.0	nan	12\|68\|68\|230\|286\|SC16TSA17C\|502.0	nan	nan	nan	GGATTCACTTTCAGTAACTACTGG	38	ATGAACTGGGTCCGCCAGTCTCCAGAGAAGGGGCTTGAGTGGGTTGCTGAA	38	ATTAGATTGAAATCTAATAATTATGCAACA	38	CATTATGCGGAGTCTGTGAAAGGGAGGTTCACCATCTCAAGAGATGATTCCAAAAGTAGTGTCTACCTGCAAATGAACAACTTAAGAGCTGAAGACACTGGCATTTATTAC	38	TGTACCAGGGCGGGAAACTCGGCCTGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFSNYW	MNWVRQSPEKGLEWVAE	IRLKSNNYAT	HYAESVKGRFTISRDDSKSSVYLQMNNLRAEDTGIYY	CTRAGNSAWFAYW	GQGTLVTVSA_	:::::0:24:75:105:216:-3:221:::::230:8:255:286::
34	886	0.00200557	GGCTACACCTTCACTACCTATCCAATAGAGTGGATGAAGCAGAATCATGGGAAGAGCCTAGAGTGGATTGGAAATTTTCATCCTTACAATGATGATACTAAGTACAATGAAAAATTCAAGGGCAAGGCCAAATTGACTGTAGAAAAATCCTCTAGCACAGTCTACTTGGAGCTCAGCCGATTAACATCTGATGACTCTGCTGTTTATTACTGTGCAAGGCACCCCTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-47*00(2523.7)	nan	IGHJ3*00(370)	IGHG2C*00(64.3)	232\|452\|471\|0\|220\|ST255ASA282GSG345ASC362A\|2084.0	nan	31\|68\|68\|225\|262\|\|370.0	nan	nan	nan	GGCTACACCTTCACTACCTATCCA	38	ATAGAGTGGATGAAGCAGAATCATGGGAAGAGCCTAGAGTGGATTGGAAAT	38	TTTCATCCTTACAATGATGATACT	38	AAGTACAATGAAAAATTCAAGGGCAAGGCCAAATTGACTGTAGAAAAATCCTCTAGCACAGTCTACTTGGAGCTCAGCCGATTAACATCTGATGACTCTGCTGTTTATTAC	38	TGTGCAAGGCACCCCTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYTFTTYP	IEWMKQNHGKSLEWIGN	FHPYNDDT	KYNEKFKGKAKLTVEKSSSTVYLELSRLTSDDSAVYY	CARHPYW	GQGTLVTVSA_	:::::0:24:75:99:210:1:220:::::225:-11:231:262::
35	885	0.00200331	GAATACGAATTCCCTTCCCATGACATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCCATTAATAGTGATGGTGGTAGCACCTACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTACTGTGCAAGACATGGTAACTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-2*00(2757.5)	IGHD2-300(25),IGHD2-700(25),IGHD2-8*00(25)	IGHJ4*00(520)	IGHM*00(64.8)	232\|455\|473\|0\|223\|\|2230.0	3\|8\|51\|223\|228\|\|25.0;3\|8\|51\|223\|228\|\|25.0;26\|31\|51\|223\|228\|\|25.0	22\|74\|74\|228\|280\|\|520.0	nan	nan	nan	GAATACGAATTCCCTTCCCATGAC	38	ATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCC	38	ATTAATAGTGATGGTGGTAGCACC	38	TACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTAC	38	TGTGCAAGACATGGTAACTACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	EYEFPSHD	MSWVRKTPEKRLELVAA	INSDGGST	YYPDTMERRFIISRDNTKKTLYLQMSSLRSEDTALYY	CARHGNYYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:2:223:223:14:-26:228:228:-2:249:280::
36	879	0.00198973	GGATTCACTTTCAGTAGCTATACCATGTCTTGGGTTCGCCAGACTCCAGAGAAGAGGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTACTATCCAGACACTGTAAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACGGCCATGTATTACTGTGCAAGACATTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-12*00(2456.9)	IGHD1-1*00(55)	IGHJ1*00(551)	IGHM*00(64.7)	232\|451\|471\|0\|219\|SG247ASA248GST253ASA254CSA260CST333CSC409ASA429G\|1958.0	26\|37\|69\|220\|231\|\|55.0	15\|73\|73\|231\|289\|SA45G\|551.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATACC	38	ATGTCTTGGGTTCGCCAGACTCCAGAGAAGAGGCTGGAGTGGGTCGCATAC	38	ATTAGTAATGGTGGTGGTAGCACC	38	TACTATCCAGACACTGTAAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGACATTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFTFSSYT	MSWVRQTPEKRLEWVAY	ISNGGGST	YYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARHYYGSSYWYFDVW	GAGTTVTVSS_	:::::0:24:75:99:210:0:219:220:-3:-9:231:231:5:258:289::
37	855	0.0019354	GGCTACACCTTTACTAGCTACACGATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGATACATTAATCCTAGCAGTGGTTATACTAATTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTGCAGACAAATCCTCCAGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATTACTGTGCAAGAAAGGACTACGGTAGTAGCTCGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-4*00(2654.7)	IGHD1-1*00(65)	IGHJ3*00(441)	IGHM*00(64.9)	232\|451\|471\|0\|219\|SG333T\|2161.0	29\|42\|69\|223\|236\|\|65.0	21\|68\|68\|236\|283\|SG23C\|441.0	nan	nan	nan	GGCTACACCTTTACTAGCTACACG	38	ATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGATAC	38	ATTAATCCTAGCAGTGGTTATACT	38	AATTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTGCAGACAAATCCTCCAGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATTAC	38	TGTGCAAGAAAGGACTACGGTAGTAGCTCGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYTFTSYT	MHWVKQRPGQGLEWIGY	INPSSGYT	NYNQKFKDKATLTADKSSSTAYMQLSSLTSEDSAVYY	CARKDYGSSSFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:223:-6:-4:236:236252:283::
38	853	0.00193087	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATATGATTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1434.6)	IGHD2-300(25),IGHD2-400(25)	IGHJ2*00(490)	IGHM*00(64.8)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1243.0	20\|25\|51\|220\|225\|\|25.0;23\|28\|51\|220\|225\|\|25.0	19\|68\|68\|225\|274\|\|490.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATATGATTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARYDYYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:220:-3:-9:225:225:1:243:274::
39	853	0.00193087	GGATACGCCTTCACTAATTACTTGATAGAGTGGGTAAAGCAGAGGCCTGGACAGGGCCTTGAGTGGATTGGAGTGATTAATCCTGGAAGTGGTGGTACTAACTACAATGAGAAGTTCAAGGGCAAGGCAACACTGACTGCAGACAAATCCTCCAGCACTGCCTACATGCAGCTCAGCAGCCTGACATCTGATGACTCTGCGGTCTATTTCTGTGCAAGATGGGATTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-54*00(2697.3)	nan	IGHJ4*00(540)	IGHM*00(64.9)	232\|452\|471\|0\|220\|SG423T\|2171.0	nan	20\|74\|74\|223\|277\|\|540.0	nan	nan	nan	GGATACGCCTTCACTAATTACTTG	38	ATAGAGTGGGTAAAGCAGAGGCCTGGACAGGGCCTTGAGTGGATTGGAGTG	38	ATTAATCCTGGAAGTGGTGGTACT	38	AACTACAATGAGAAGTTCAAGGGCAAGGCAACACTGACTGCAGACAAATCCTCCAGCACTGCCTACATGCAGCTCAGCAGCCTGACATCTGATGACTCTGCGGTCTATTTC	38	TGTGCAAGATGGGATTACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYAFTNYL	IEWVKQRPGQGLEWIGV	INPGSGGT	NYNEKFKGKATLTADKSSSTAYMQLSSLTSDDSAVYF	CARWDYYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:1:220:::::223:0:246:277::
40	843	0.00190824	GGGTTCTCATTATCCAGATATAGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAATGATATGGGGTGGTGGAAGCACAGACTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTACTGTGCCAGAAATGATTACGACTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-6-800(2359.6),IGHV2-600(2311.7)	IGHD2-4*00(50)	IGHJ3*00(470)	IGHM*00(64.8)	232\|447\|470\|0\|215\|SA244TSC249ASG253ASG259CST274CSG304ASA306GST317GSA328GST330C\|1860.0;232\|448\|468\|0\|216\|SA244TSC249ASG253AST302GSG304ASA313GSA317GSA328GSC329ASC411G\|1870.0	23\|33\|51\|217\|227\|\|50.0	21\|68\|68\|227\|274\|\|470.0	nan	nan	nan	GGGTTCTCATTATCCAGATATAGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAATG	38	ATATGGGGTGGTGGAAGCACA	38	GACTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGAAATGATTACGACTGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFSLSRYS	VHWVRQPPGKGLEWLGM	IWGGGST	DYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CARNDYDWFAYW	GQGTLVTVSA_	:::::0:24:75:96:207:-3:215:217:-6:-1:227:227243:274::
41	825	0.00186749	GGGTTTTCATTAACCAGCTATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTAATATGGGCTGGTGGAAGCACAAATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTACTGTGCCAGAGAGGGGGATCTACTATGGTTACGACATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-9*00(2580.5)	IGHD2-700(95),IGHD2-400(81)	IGHJ4*00(480)	IGHM*00(64.8)	232\|446\|469\|0\|214\|SG259CSG314CSA339G\|2053.0	15\|34\|51\|222\|241\|\|95.0;15\|34\|51\|222\|241\|SA26G\|81.0	26\|74\|74\|241\|289\|\|480.0	nan	nan	nan	GGGTTTTCATTAACCAGCTATGGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTA	38	ATATGGGCTGGTGGAAGCACA	38	AATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGAGAGGGGGATCTACTATGGTTACGACATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQPPGKGLEWLGV	IWAGGST	NYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CAREGDLLWLRHAMDYW	GQGTSVTVSS_	:::::0:24:75:96:207:-3:214:222:2:0:241:241:-6:258:289::
42	769	0.00174073	GGCTTCAACATTAAAGACACCTATATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGGATTGATCCTGCGAATGGTAATACTAAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTACTGTGCTAGAAATGGTAACGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV14-3*00(2543.5)	IGHD2-8*00(40)	IGHJ3*00(400)	IGHM*00(64.3)	232\|451\|471\|0\|219\|SA247GSC338AST369ASA433G\|2074.0	23\|31\|51\|220\|228\|\|40.0	28\|68\|68\|228\|268\|\|400.0	nan	nan	nan	GGCTTCAACATTAAAGACACCTAT	38	ATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGG	38	ATTGATCCTGCGAATGGTAATACT	38	AAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTAC	38	TGTGCTAGAAATGGTAACGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFNIKDTY	MHWVKQRPEQGLEWIGR	IDPANGNT	KYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYY	CARNGNAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:220:-6:-3:228:228:-8:237:268::
43	766	0.00173394	GGCTACTCCTTCACCAGCTACTGGATGAACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGCATGATTCATCCTTCCGATAGTGAAACTAGGTTAAATCAGAAGTTCAAGGACAAGGCCACATTGACTGTAGACAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCCCGACATCTGAGGACTCTGCGGTCTATTACTGTGCAAGAGGGGACTATAGGTACGGGGTGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-7400(2147.3),IGHV1-6100(2117.3)	IGHD2-500(35),IGHD2-600(35),IGHD5-1*00(35)	IGHJ4*00(420)	IGHG2C*00(63.2)	232\|449\|471\|0\|217\|SA238TSC259ASC282ASA303CSG305TST318CST327ASA332GSC333GSA335TSC336ASA342GSG353ASG402AST413C\|1735.0;232\|451\|471\|0\|219\|SA238TSG259AST261CSA294GST303CSA305TSC306GST310CSC312TST318CSC331ASA332GSC333GSA335TSC336ASA342GSG402AST413C\|1668.0	6\|13\|51\|223\|230\|\|35.0;6\|13\|51\|223\|230\|\|35.0;1\|8\|30\|227\|234\|\|35.0	32\|74\|74\|238\|280\|\|420.0	nan	nan	nan	GGCTACTCCTTCACCAGCTACTGG	38	ATGAACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGCATG	38	ATTCATCCTTCCGATAGTGAAACT	38	AGGTTAAATCAGAAGTTCAAGGACAAGGCCACATTGACTGTAGACAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCCCGACATCTGAGGACTCTGCGGTCTATTAC	38	TGTGCAAGAGGGGACTATAGGTACGGGGTGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYSFTSYW	MNWVKQRPGQGLEWIGM	IHPSDSET	RLNQKFKDKATLTVDKSSSTAYMQLSSPTSEDSAVYY	CARGDYRYGVDYW	GQGTSVTVSS_	:::::0:24:75:99:210:-2:217:223:11:-21:230:238:-12:249:280::
44	751	0.00169998	GGCTACACCTTCACCAGCTACTATATGTACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGAGAGATTAATCCTAGCAATGGTGGTACTAACTTCAATGAGAAGTTCAAGAGCAAGGCCACACTGACTGTAGACAAATCCTCCAGCACAGCATACATGCAACTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTACTGTACAAGAAGGAGGAATTACTACGGTAGTAGCTACGGAGGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-53*00(2368.7)	IGHD1-1*00(106)	IGHJ3*00(450)	IGHM*00(64.7)	232\|451\|471\|0\|219\|SG254ASG255TSC259TSA304GST306GSA335TSC393ASG402AST441CSG445A\|1900.0	26\|50\|69\|226\|250\|ST47G\|106.0	23\|68\|68\|250\|295\|\|450.0	nan	nan	nan	GGCTACACCTTCACCAGCTACTAT	38	ATGTACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGAGAG	38	ATTAATCCTAGCAATGGTGGTACT	38	AACTTCAATGAGAAGTTCAAGAGCAAGGCCACACTGACTGTAGACAAATCCTCCAGCACAGCATACATGCAACTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTAC	38	TGTACAAGAAGGAGGAATTACTACGGTAGTAGCTACGGAGGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYTFTSYY	MYWVKQRPGQGLEWIGE	INPSNGGT	NFNEKFKSKATLTVDKSSSTAYMQLSSLTSEDSAVYY	CTRRRNYYGSSYGGFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:226:-3:4:250:250:-3:264:295::
45	690	0.0015619	GGATTCACTTTCAGTAGCTATGGCATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGACTAACTGGTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2715.6)	IGHD4-1*00(40)	IGHJ3*00(410)	IGHM*00(64.7)	232\|451\|471\|0\|219\|\|2190.0	11\|19\|33\|219\|227\|\|40.0	27\|68\|68\|227\|268\|\|410.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGGC	38	ATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGACTAACTGGTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFSSYG	MSWVRQTPDKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARLTGAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:219:0:-3:227:227:-7:237:268::
46	683	0.00154606	GGGTTTTCATTAACCAGCTATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTAATATGGGCTGGTGGAAGCACAAATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTACTGTGCCAGAGGGTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-9*00(2580.7)	IGHD1-1*00(45)	IGHJ1*00(551)	IGHM*00(64.7)	232\|446\|469\|0\|214\|SG259CSG314CSA339G\|2053.0	28\|37\|69\|219\|228\|\|45.0	15\|73\|73\|228\|286\|SA45G\|551.0	nan	nan	nan	GGGTTTTCATTAACCAGCTATGGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTA	38	ATATGGGCTGGTGGAAGCACA	38	AATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGAGGGTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQPPGKGLEWLGV	IWAGGST	NYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CARGYYGSSYWYFDVW	GAGTTVTVSS_	:::::0:24:75:96:207:-3:214:219:-5:-9:228:228:5:255:286::
47	682	0.00154379	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATGGGGGTACTACGGTAGTAGCTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1437.5)	IGHD1-1*00(70)	IGHJ4*00(500)	IGHM*00(64.9)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1243.0	28\|42\|69\|225\|239\|\|70.0	24\|74\|74\|239\|289\|\|500.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATGGGGGTACTACGGTAGTAGCTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARWGYYGSSYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:1:220:225:-5:-4:239:239:-4:258:289::
48	679	0.001537	GGCTACACCTTCACTGACCATGCTATTCACTGGGTGAAGCAGAAGCCTGAACAGGGCCTGGAATGGATTGGATATATTTCTCCCGGAAATGGTGATATTAAGTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACTGCCTACATGCAGCTCAACAGCCTGACATCTGAGGATTCTGCAGTGTATTTCTGTAAAAGATGGGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-78*00(2202.3)	nan	IGHJ2*00(400)	IGHM*00(64.8)	232\|452\|471\|0\|220\|SA253GSA265GSG275ASA311CST315CSA316GSG319ASA325GSG326ASC329TST364CSA390TSC426TSC435GSG445ASC446A\|1736.0	nan	28\|68\|68\|222\|262\|\|400.0	nan	nan	nan	GGCTACACCTTCACTGACCATGCT	38	ATTCACTGGGTGAAGCAGAAGCCTGAACAGGGCCTGGAATGGATTGGATAT	38	ATTTCTCCCGGAAATGGTGATATT	38	AAGTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACTGCCTACATGCAGCTCAACAGCCTGACATCTGAGGATTCTGCAGTGTATTTC	38	TGTAAAAGATGGGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDHA	IHWVKQKPEQGLEWIGY	ISPGNGDI	KYNEKFKGKATLTADKSSSTAYMQLNSLTSEDSAVYF	CKRWDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:::::222:-8:231:262::
49	666	0.00150758	GGATTCACTTTCAGTAGCTTTGGAATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATACATTAGTAGTGGCAGTAGTACCATCTACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTACTGTGCAAGATACTATGGTAATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-17*00(2478.7)	IGHD2-800(50),IGHD2-700(45)	IGHJ4*00(480)	IGHM*00(64.9)	232\|450\|471\|0\|218\|SG247ASA248GSA251TST300CSG379CSG414A\|2006.0	19\|29\|51\|219\|229\|\|50.0;19\|28\|51\|219\|228\|\|45.0	26\|74\|74\|229\|277\|\|480.0	nan	nan	nan	GGATTCACTTTCAGTAGCTTTGGA	38	ATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATAC	38	ATTAGTAGTGGCAGTAGTACCATC	38	TACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGATACTATGGTAATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GFTFSSFG	MHWVRQAPEKGLEWVAY	ISSGSSTI	YYADTVKGRFTISRDNPKNTLFLQMTSLRSEDTAMYY	CARYYGNAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:-1:218:219:-2:-5:229:229:-6:246:277::
50	615	0.00139213	GGATTCACTTTCAGTAGCTTTGGAATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATACATTAGTAGTGGCAGTAGTACCATCTACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTACTGTGCAAGATACTATGGTAACTACGCCTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-17*00(2477.9)	IGHD2-8*00(80)	IGHJ3*00(430)	IGHM*00(64.9)	232\|450\|471\|0\|218\|SG247ASA248GSA251TST300CSG379CSG414A\|2006.0	19\|35\|51\|219\|235\|\|80.0	25\|68\|68\|237\|280\|\|430.0	nan	nan	nan	GGATTCACTTTCAGTAGCTTTGGA	38	ATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATAC	38	ATTAGTAGTGGCAGTAGTACCATC	38	TACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGATACTATGGTAACTACGCCTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFSSFG	MHWVRQAPEKGLEWVAY	ISSGSSTI	YYADTVKGRFTISRDNPKNTLFLQMTSLRSEDTAMYY	CARYYGNYAFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:-1:218:219:-2:1:235:237:-5:249:280::
51	609	0.00137855	GGCTACTCAATCACCAGTGATTATGCCTGGAACTGGATCCGGCAGTTTCCAGGAAACAAACTGGAGTGGATGGGCTACATAAGCTACAGTGGTAGCACTAGCTACAACCCATCTCTCAAAAGTCGAATCTCTATCACTCGAGACACATCCAAGAACCAGTTCTTCCTGCAGTTGAATTCTGTGACTACTGAGGACACAGCCACATATTACTGTGCAAGATTTCTCTATGATGGTTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV3-100(2235.6),IGHV3-600(2204.7)	IGHD2-3*00(55)	IGHJ2*00(490)	IGHM*00(64.9)	229\|449\|468\|0\|220\|SC237ASG248ASA254CSC259ASA270GST273GSA329GSC342TSC360TSA368GST369AST378CST387GSA397C\|1794.0;229\|455\|470\|0\|226\|SC237ASG248AST253GSA254CSA294GSG316ASA317GSA326CSA329GSA350GSC360TST369AST378CST390CSA397CSG448TSA449T\|1767.0	17\|28\|51\|223\|234\|\|55.0	19\|68\|68\|234\|283\|\|490.0	nan	nan	nan	GGCTACTCAATCACCAGTGATTATGCC	38	TGGAACTGGATCCGGCAGTTTCCAGGAAACAAACTGGAGTGGATGGGCTAC	38	ATAAGCTACAGTGGTAGCACT	38	AGCTACAACCCATCTCTCAAAAGTCGAATCTCTATCACTCGAGACACATCCAAGAACCAGTTCTTCCTGCAGTTGAATTCTGTGACTACTGAGGACACAGCCACATATTAC	38	TGTGCAAGATTTCTCTATGATGGTTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYSITSDYA	WNWIRQFPGNKLEWMGY	ISYSGST	SYNPSLKSRISITRDTSKNQFFLQLNSVTTEDTATYY	CARFLYDGYYFDYW	GQGTTLTVSS_	:::::0:27:78:99:210:1:220:223:0:-6:234:234:1:252:283::
52	598	0.00135365	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATTGGACGGTAGTAGCTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1428.1)	IGHD1-1*00(50)	IGHJ2*00(470)	IGHM*00(64.8)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1243.0	32\|42\|69\|223\|233\|\|50.0	21\|68\|68\|233\|280\|\|470.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATTGGACGGTAGTAGCTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARLDGSSYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:223:-9:-4:233:233249:280::
53	586	0.00132649	GAATACGAATTCCCTTCCCATGACATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCCATTAATAGTGATGGTGGTAGCACCTACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTACTGTGCAAGACATGGTAACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-2*00(2757.3)	nan	IGHJ2*00(480)	IGHM*00(64.9)	232\|455\|473\|0\|223\|\|2230.0	nan	20\|68\|68\|226\|274\|\|480.0	nan	nan	nan	GAATACGAATTCCCTTCCCATGAC	38	ATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCC	38	ATTAATAGTGATGGTGGTAGCACC	38	TACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTAC	38	TGTGCAAGACATGGTAACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	EYEFPSHD	MSWVRKTPEKRLELVAA	INSDGGST	YYPDTMERRFIISRDNTKKTLYLQMSSLRSEDTALYY	CARHGNYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:2:223:::::226:0:243:274::
54	504	0.00114087	GGATTCACCTTCAATACCTACGCCATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGTTTGGAATGGGTTGCTCGCATAAGAAGTAAAAGTAATAATTATGCAACATATTATGCCGATTCAGTGAAAGACAGGTTCACCATCTCCAGAGATGATTCACAAAGCATGCTCTATCTGCAAATGAACAACTTGAAAACTGAGGACACAGCCATGTATTACTGTGTAGCTAACTGGGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV10-1*00(2648)	IGHD4-1*00(50)	IGHJ2*00(400)	IGHM*00(64.7)	232\|453\|477\|0\|221\|SG239CSA363GSG388C\|2123.0	9\|19\|33\|221\|231\|\|50.0	28\|68\|68\|231\|271\|\|400.0	nan	nan	nan	GGATTCACCTTCAATACCTACGCC	38	ATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGTTTGGAATGGGTTGCTCGC	38	ATAAGAAGTAAAAGTAATAATTATGCAACA	38	TATTATGCCGATTCAGTGAAAGACAGGTTCACCATCTCCAGAGATGATTCACAAAGCATGCTCTATCTGCAAATGAACAACTTGAAAACTGAGGACACAGCCATGTATTAC	38	TGTGTAGCTAACTGGGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFNTYA	MNWVRQAPGKGLEWVAR	IRSKSNNYAT	YYADSVKDRFTISRDDSQSMLYLQMNNLKTEDTAMYY	CVANWDYW	GQGTTLTVSS_	:::::0:24:75:105:216:-4:221:221:2:-3:231:231:-8:240:271::
55	494	0.00111823	GAATACGAATTCCCTTCCCATGACATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCCATTAATAGTGATGGTGGTAGCACCTACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTACTGTGCAAGACATGCCCGATATTACTACGGTAGTAGCCTCTGGGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-2*00(2756.6)	IGHD1-1*00(90)	IGHJ4*00(460)	IGHM*00(64.9)	232\|455\|473\|0\|223\|\|2230.0	25\|43\|69\|228\|246\|\|90.0	28\|74\|74\|252\|298\|\|460.0	nan	nan	nan	GAATACGAATTCCCTTCCCATGAC	38	ATGTCTTGGGTCCGCAAGACTCCGGAGAAGAGGCTGGAGTTGGTCGCAGCC	38	ATTAATAGTGATGGTGGTAGCACC	38	TACTATCCAGACACCATGGAGAGACGATTCATCATCTCCAGAGACAATACCAAGAAGACCCTGTACCTGCAAATGAGCAGTCTGAGGTCTGAGGACACAGCCTTGTATTAC	38	TGTGCAAGACATGCCCGATATTACTACGGTAGTAGCCTCTGGGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	EYEFPSHD	MSWVRKTPEKRLELVAA	INSDGGST	YYPDTMERRFIISRDNTKKTLYLQMSSLRSEDTALYY	CARHARYYYGSSLWAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:2:223:228:-2:-3:246:252:-8:267:298::
56	492	0.0011137	GGATTCACTTTCAGTAGCTATGGCATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGACATGATTACGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2714.7)	IGHD2-4*00(40)	IGHJ2*00(400)	IGHM*00(64.9)	232\|451\|471\|0\|219\|\|2190.0	23\|31\|51\|220\|228\|\|40.0	28\|68\|68\|228\|268\|\|400.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGGC	38	ATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGACATGATTACGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYG	MSWVRQTPDKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARHDYDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:220:-6:-3:228:228:-8:237:268::
57	491	0.00111144	GGATTCACTTTCAGTAGCTATACCATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTACCTATGGTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2482.9)	IGHD2-700(30),IGHD2-800(30)	IGHJ2*00(410)	IGHM*00(64.9)	232\|445\|471\|0\|213\|SG253ASG254CSA279GSC282GSG354C\|1985.0	21\|27\|51\|215\|221\|\|30.0;21\|27\|51\|215\|221\|\|30.0	27\|68\|68\|221\|262\|\|410.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATACC	38	ATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTACCTATGGTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYT	MSWVRQTPEKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CTYGDYW	GQGTTLTVSS_	:::::0:24:75:99:210:-6:213:215:-4:-7:221:221:-7:231:262::
58	490	0.00110918	GGATTCACCTTCAATACCTACGCCATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGTTTGGAATGGGTTGCTCGCATAAGAAGTAAAAGTAATAATTATGCAACATATTATGCCGATTCAGTGAAAGACAGGTTCACCATCTCCAGAGATGATTCACAAAGCATGCTCTATCTGCAAATGAACAACTTGAAAACTGAGGACACAGCCATGTATTACTGTGTGAGACACGAACTACGTTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV10-1*00(2688.5)	IGHD2-500(40),IGHD2-600(40),IGHD2-8*00(40)	IGHJ1*00(491)	IGHM*00(64.8)	232\|457\|477\|0\|225\|SG239CSA363GSG388C\|2163.0	28\|36\|51\|229\|237\|\|40.0;28\|36\|51\|229\|237\|\|40.0;28\|36\|51\|229\|237\|\|40.0	21\|73\|73\|237\|289\|SA45G\|491.0	nan	nan	nan	GGATTCACCTTCAATACCTACGCC	38	ATGAACTGGGTCCGCCAGGCTCCAGGAAAGGGTTTGGAATGGGTTGCTCGC	38	ATAAGAAGTAAAAGTAATAATTATGCAACA	38	TATTATGCCGATTCAGTGAAAGACAGGTTCACCATCTCCAGAGATGATTCACAAAGCATGCTCTATCTGCAAATGAACAACTTGAAAACTGAGGACACAGCCATGTATTAC	38	TGTGTGAGACACGAACTACGTTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFTFNTYA	MNWVRQAPGKGLEWVAR	IRSKSNNYAT	YYADSVKDRFTISRDDSQSMLYLQMNNLKTEDTAMYY	CVRHELRYWYFDVW	GAGTTVTVSS_	:::::0:24:75:105:216:0:225:229:-11:2:237:237258:289::
59	486	0.00110012	GGATTCACTTTCAGTAGCTTTGGAATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATACATTAGTAGTGGCAGTAGTACCATCTACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTACTGTGCAAGACTGGGACTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-17*00(2477.6)	IGHD4-1*00(40)	IGHJ2*00(420)	IGHM*00(64.8)	232\|450\|471\|0\|218\|SG247ASA248GSA251TST300CSG379CSG414A\|2006.0	14\|22\|33\|218\|226\|\|40.0	26\|68\|68\|226\|268\|\|420.0	nan	nan	nan	GGATTCACTTTCAGTAGCTTTGGA	38	ATGCACTGGGTTCGTCAGGCTCCAGAGAAGGGGCTGGAGTGGGTCGCATAC	38	ATTAGTAGTGGCAGTAGTACCATC	38	TACTATGCAGACACAGTGAAGGGCCGATTCACCATCTCCAGAGACAATCCCAAGAACACCCTGTTCCTGCAAATGACCAGTCTAAGGTCTGAGGACACGGCCATGTATTAC	38	TGTGCAAGACTGGGACTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSFG	MHWVRQAPEKGLEWVAY	ISSGSSTI	YYADTVKGRFTISRDNPKNTLFLQMTSLRSEDTAMYY	CARLGLDYW	GQGTTLTVSS_	:::::0:24:75:99:210:-1:218:218:-3:0:226:226:-6:237:268::
60	479	0.00108428	GGATTCACTTTCAGTAGCTATGGCATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGAGGGTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2714.6)	nan	IGHJ2*00(430)	IGHM*00(64.8)	232\|451\|471\|0\|219\|\|2190.0	nan	25\|68\|68\|222\|265\|\|430.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGGC	38	ATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGAGGGTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYG	MSWVRQTPDKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARGFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:::::222:-5:234:265::
61	475	0.00107522	GGGTTCTCATTAACCAGCTATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTCCAAACTGATGACACAGCCATGTACTACTGTGCCAGACAGGCCTACTATGGTAACTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-6*00(2659.8)	IGHD2-500(55),IGHD2-600(55)	IGHJ1*00(512)	IGHM*00(64.9)	232\|448\|468\|0\|216\|\|2160.0	14\|25\|51\|217\|228\|\|55.0;14\|25\|51\|217\|228\|\|55.0	16\|73\|73\|229\|286\|SG19ASA45G\|512.0	nan	nan	nan	GGGTTCTCATTAACCAGCTATGGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTG	38	ATATGGAGTGATGGAAGCACA	38	ACCTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTCCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGACAGGCCTACTATGGTAACTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQPPGKGLEWLVV	IWSDGST	TYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CARQAYYGNYWYFDVW	GAGTTVTVSS_	:::::0:24:75:96:207:0:216:217:3:-9:228:229:4:255:286::
62	468	0.00105938	GGTTACTCATTCACTGACTACAACATGTACTGGGTGAAGCAGAGCCATGGAAAGAGCCTTGAGTGGATTGGATATATTGATCCTTACAATGGTGGTACTAGCTACAACCAGAAGTTCAAGGGCAAGGCCACATTGACTGTTGACAAGTCCTCCAGCACAGCCTTCATGCATCTCAACAGCCTGACATCTGAGGACTCTGCAGTCTATTACTGTGCAAGATCAGGGGGCTACAGTAGCCTACGGGGTGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-1900(2273.8),IGHV1-1800(2252.7),IGHV1-26*00(2247.5)	IGHD3-100(53),IGHD3-200(53)	IGHJ4*00(470)	IGHG2C*00(62.8)	232\|453\|471\|0\|221\|SA234TSA238TST253AST255CSA259TSG304TST305ASA310GSC321TSA395TSG400CSG402T\|1862.0;232\|453\|471\|0\|221\|SA234TSA238TSG259TSG304TSA310GSA316TST332GSA372TSA395TSG400CSG402TSC406ASG407ASA427T\|1804.0;232\|453\|471\|0\|221\|SA234TSA238TSG240AST253ASA259TSG304TSA310GSA316TSA372TSA395TSG400CSG402TSC406ASG407A\|1804.0	27\|40\|51\|224\|238\|I34A\|53.0;26\|39\|48\|225\|239\|I32A\|53.0	27\|74\|74\|245\|292\|\|470.0	nan	nan	nan	GGTTACTCATTCACTGACTACAAC	38	ATGTACTGGGTGAAGCAGAGCCATGGAAAGAGCCTTGAGTGGATTGGATAT	38	ATTGATCCTTACAATGGTGGTACT	38	AGCTACAACCAGAAGTTCAAGGGCAAGGCCACATTGACTGTTGACAAGTCCTCCAGCACAGCCTTCATGCATCTCAACAGCCTGACATCTGAGGACTCTGCAGTCTATTAC	38	TGTGCAAGATCAGGGGGCTACAGTAGCCTACGGGGTGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYSFTDYN	MYWVKQSHGKSLEWIGY	IDPYNGGT	SYNQKFKGKATLTVDKSSSTAFMHLNSLTSEDSAVYY	CARSGGYSSLRGAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:2:221:224:-10:6:238:245:-7:261:292::
63	459	0.001039	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATCGGGGGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1441.1)	nan	IGHJ4*00(460)	IGHM*00(64.9)	232\|453\|471\|0\|221\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1253.0	nan	28\|74\|74\|225\|271\|\|460.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATCGGGGGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARSGAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:2:221:::::225:-8:240:271::
64	442	0.00100052	GGCTACTCAATCACCAGTGATTATGCCTGGAACTGGATCCGGCAGTTTCCAGGAAACAAACTGGAGTGGATGGGCTACATAAGCTACAGTGGTAGCACTAGCTACAACCCATCTCTCAAAAGTCGAATCTCTATCACTCGAGACACATCCAAGAACCAGTTCTTCCTGCAGTTGAATTCTGTGACTACTGAGGACACAGCCACATATTACTGTGCAAGATGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV3-100(2235.5),IGHV3-600(2190.9)	nan	IGHJ3*00(460)	IGHM*00(65)	229\|449\|468\|0\|220\|SC237ASG248ASA254CSC259ASA270GST273GSA329GSC342TSC360TSA368GST369AST378CST387GSA397C\|1794.0;229\|448\|470\|0\|219\|SC237ASG248AST253GSA254CSA294GSG316ASA317GSA326CSA329GSA350GSC360TST369AST378CST390CSA397C\|1755.0	nan	22\|68\|68\|219\|265\|\|460.0	nan	nan	nan	GGCTACTCAATCACCAGTGATTATGCC	38	TGGAACTGGATCCGGCAGTTTCCAGGAAACAAACTGGAGTGGATGGGCTAC	38	ATAAGCTACAGTGGTAGCACT	38	AGCTACAACCCATCTCTCAAAAGTCGAATCTCTATCACTCGAGACACATCCAAGAACCAGTTCTTCCTGCAGTTGAATTCTGTGACTACTGAGGACACAGCCACATATTAC	38	TGTGCAAGATGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYSITSDYA	WNWIRQFPGNKLEWMGY	ISYSGST	SYNPSLKSRISITRDTSKNQFFLQLNSVTTEDTATYY	CARWFAYW	GQGTLVTVSA_	:::::0:27:78:99:210:1:220:::::219:-2:234:265::
65	434	0.000982414	GGGTTCTCATTAACCGGCTATGGTGTAAACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAATGATATGGGGTGATGGAAGCACAGACTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCAGGTACTACTGTGCCAGAGATGGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-600(2426.3),IGHV2-6-800(2369.6)	nan	IGHJ3*00(450)	IGHM*00(64.7)	232\|448\|468\|0\|216\|SA247GSC259AST302GSG304ASA313GSA328GSC329ASC411GST431G\|1899.0;232\|451\|470\|0\|219\|SA247GSG259AST274CSG304ASA306GST317ASA328GST330CST431GST447A\|1900.0	nan	23\|68\|68\|220\|265\|\|450.0	nan	nan	nan	GGGTTCTCATTAACCGGCTATGGT	38	GTAAACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAATG	38	ATATGGGGTGATGGAAGCACA	38	GACTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCAGGTACTAC	38	TGTGCCAGAGATGGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFSLTGYG	VNWVRQPPGKGLEWLGM	IWGDGST	DYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTARYY	CARDGFAYW	GQGTLVTVSA_	:::::0:24:75:96:207:0:216:::::220:-3:234:265::
66	430	0.000973359	GGTTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCCAGTCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTGATATGGAGAGGTGGAAGCACAGACTACAATGCAGCTTTCATGTCCAGACTGAGCATCACCAAGGACAACTCCAAGAGCCAAGTTTTCTTTAAAATGAACAGTCTGCAAGCTGATGACACTGCCATATACTACTGTGCCAAATATGGTAACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-5*00(2681.5)	nan	IGHJ4*00(510)	IGHM*00(64.8)	232\|451\|470\|0\|219\|SA448T\|2161.0	nan	23\|74\|74\|223\|274\|\|510.0	nan	nan	nan	GGTTTCTCATTAACTAGCTATGGT	38	GTACACTGGGTTCGCCAGTCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTG	38	ATATGGAGAGGTGGAAGCACA	38	GACTACAATGCAGCTTTCATGTCCAGACTGAGCATCACCAAGGACAACTCCAAGAGCCAAGTTTTCTTTAAAATGAACAGTCTGCAAGCTGATGACACTGCCATATACTAC	38	TGTGCCAAATATGGTAACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQSPGKGLEWLGV	IWRGGST	DYNAAFMSRLSITKDNSKSQVFFKMNSLQADDTAIYY	CAKYGNYAMDYW	GQGTSVTVSS_	:::::0:24:75:96:207:1:219:::::223:-3:243:274::
67	425	0.000962041	GGATTCACTTTCAGTAGCTATACCATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTACAAGAGATGGTTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2514.7)	IGHD2-3*00(30)	IGHJ2*00(370)	IGHM*00(64.9)	232\|451\|471\|0\|219\|SG253ASG254CSA279GSC282GSG354CSG445A\|2016.0	22\|28\|51\|219\|225\|\|30.0	31\|68\|68\|225\|262\|\|370.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATACC	38	ATGTCTTGGGTTCGCCAGACTCCGGAGAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTACAAGAGATGGTTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYT	MSWVRQTPEKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CTRDGYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:219:-5:-6:225:225:-11:231:262::
68	422	0.00095525	GGCTTCAACATTAAAGACACCTATATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGGATTGATCCTGCGAATGGTAATACTAAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTACTGTGCTAGGGTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV14-3*00(2533.6)	nan	IGHJ2*00(420)	IGHM*00(65)	232\|450\|471\|0\|218\|SA247GSC338AST369ASA433G\|2064.0	nan	26\|68\|68\|220\|262\|\|420.0	nan	nan	nan	GGCTTCAACATTAAAGACACCTAT	38	ATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGG	38	ATTGATCCTGCGAATGGTAATACT	38	AAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTAC	38	TGTGCTAGGGTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFNIKDTY	MHWVKQRPEQGLEWIGR	IDPANGNT	KYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYY	CARVDYW	GQGTTLTVSS_	:::::0:24:75:99:210:-1:218:::::220:-6:231:262::
69	420	0.000950723	GGGTTCACCTTCACTGATTACTACATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTTATTAGAAACAAAGCTAATGGTTACACAACAGAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTACTGTGCAAGAGATGGGCTAAGGGCCCTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV7-3*00(2605.3)	IGHD3-1*00(30)	IGHJ3*00(420)	IGHG2C*00(56.6)	232\|460\|481\|0\|228\|SA234GSG282AST414CSG415AST457G\|2135.0	27\|33\|51\|228\|234\|\|30.0	26\|68\|68\|241\|283\|\|420.0	nan	nan	nan	GGGTTCACCTTCACTGATTACTAC	38	ATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTT	38	ATTAGAAACAAAGCTAATGGTTACACAACA	38	GAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTAC	38	TGTGCAAGAGATGGGCTAAGGGCCCTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFTDYY	MSWVRQPPGKALEWLGF	IRNKANGYTT	EYSASVKGRFTISRDNSQSILYLQMNTLRAEDSATYY	CARDGLRALAYW	GQGTLVTVSA_	:::::0:24:75:105:216:-1:228:228:-10:-1:234:241:-6:252:283::
70	419	0.000948459	GGCTACACCTTCACCAGCTACTATATGTACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGGGGGATTAATCCTAGCAATGGTGGTACTAACTTCAATGAGAAGTTCAAGAGCAAGGCCACACTGACTGTAGACAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTACTGTACAAGTAACTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-53*00(2330.1)	nan	IGHJ3*00(470)	IGHM*00(64.8)	232\|450\|471\|0\|218\|SG254ASG255TSC259TSA303GSA304GSA305GST306GSA335TSG402AST441CSG445A\|1861.0	nan	21\|68\|68\|221\|268\|\|470.0	nan	nan	nan	GGCTACACCTTCACCAGCTACTAT	38	ATGTACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGGGGG	38	ATTAATCCTAGCAATGGTGGTACT	38	AACTTCAATGAGAAGTTCAAGAGCAAGGCCACACTGACTGTAGACAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTAC	38	TGTACAAGTAACTGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYTFTSYY	MYWVKQRPGQGLEWIGG	INPSNGGT	NFNEKFKSKATLTVDKSSSTAYMQLSSLTSEDSAVYY	CTSNWFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:-1:218:::::221:-1:237:268::
71	419	0.000948459	GGATTCACTTTTAGTGACGCCTGGATGGACTGGGTCCGCCAGTCTCCAGAGAAGGGGCTTGAGTGGGTTGCTGAAATTAGAAGCAAAGCTAATAATCATGCAACATACTATGCTGAGTCTGTGAAAGGGAGGTTCACCATCTCAAGAGATGATTCCAAAAGTAGTGTCTACCTGCAAATGAACAGCTTAAGAGCTGAAGACACTGGCATTTATTACTGTACCAACTATAGGTACGACTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV6-6*00(2728.5)	IGHD2-700(37),IGHD2-600(35),IGHD5-1*00(35)	IGHJ3*00(470)	IGHG2C*00(63.6)	232\|455\|477\|0\|223\|SA314G\|2201.0	20\|33\|51\|223\|236\|SG25AST27G\|37.0;6\|13\|51\|223\|230\|\|35.0;1\|8\|30\|227\|234\|\|35.0	21\|68\|68\|236\|283\|\|470.0	nan	nan	nan	GGATTCACTTTTAGTGACGCCTGG	38	ATGGACTGGGTCCGCCAGTCTCCAGAGAAGGGGCTTGAGTGGGTTGCTGAA	38	ATTAGAAGCAAAGCTAATAATCATGCAACA	38	TACTATGCTGAGTCTGTGAAAGGGAGGTTCACCATCTCAAGAGATGATTCCAAAAGTAGTGTCTACCTGCAAATGAACAGCTTAAGAGCTGAAGACACTGGCATTTATTAC	38	TGTACCAACTATAGGTACGACTGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFSDAW	MDWVRQSPEKGLEWVAE	IRSKANNHAT	YYAESVKGRFTISRDDSKSSVYLQMNSLRAEDTGIYY	CTNYRYDWFAYW	GQGTLVTVSA_	:::::0:24:75:105:216:-2:223:223:-3:-1:236:236252:283::
72	418	0.000946196	GGATTCACTTTCAGTAGCTATGGCATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGACTAACTGGGGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2713.3)	IGHD4-1*00(45)	IGHJ3*00(400)	IGHM*00(64.8)	232\|451\|471\|0\|219\|\|2190.0	11\|20\|33\|219\|228\|\|45.0	28\|68\|68\|228\|268\|\|400.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGGC	38	ATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGACTAACTGGGGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFTFSSYG	MSWVRQTPDKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARLTGAYW	GQGTLVTVSA_	:::::0:24:75:99:210:0:219:219:0:-2:228:228:-8:237:268::
73	415	0.000939405	GGATTCACTTTCAGTAGCTATGGCATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGACACTATGGTAACTACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2714.7)	IGHD2-8*00(55)	IGHJ2*00(490)	IGHM*00(64.8)	232\|451\|471\|0\|219\|\|2190.0	20\|31\|51\|220\|231\|\|55.0	19\|68\|68\|231\|280\|\|490.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGGC	38	ATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGACACTATGGTAACTACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYG	MSWVRQTPDKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARHYGNYYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:220:-3:-3:231:231:1:249:280::
74	405	0.000916769	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACAATGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATACGGTAGTAGCTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1492.1)	IGHD1-1*00(50)	IGHJ2*00(470)	IGHM*00(64.8)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1301.0	32\|42\|69\|220\|230\|\|50.0	21\|68\|68\|230\|277\|\|470.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACAATGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATACGGTAGTAGCTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYNGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARYGSSYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:220:-9:-4:230:230246:277::
75	401	0.000907714	GGCTACACCTTCACTACCTATCCAATAGAGTGGATGAAGCAGAATCATGGGAAGAGCCTAGAGTGGATTGGAAATTTTCATCCTTACAATGATGATACTAAGTACAATGAAAAATTCAAGGGCAAGGCCAAATTGACTGTAGAAAAATCCTCTAGCACAGTCTACTTGGAGCTCAGCCGATTAACATCTGATGACTCTGCTGTTTATTACTGTGCAAGGTATGGTAACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-47*00(2512.5)	IGHD2-800(35),IGHD2-700(30)	IGHJ4*00(510)	IGHM*00(64.9)	232\|451\|471\|0\|219\|ST255ASA282GSG345ASC362A\|2074.0	22\|29\|51\|219\|226\|\|35.0;22\|28\|51\|219\|225\|\|30.0	23\|74\|74\|226\|277\|\|510.0	nan	nan	nan	GGCTACACCTTCACTACCTATCCA	38	ATAGAGTGGATGAAGCAGAATCATGGGAAGAGCCTAGAGTGGATTGGAAAT	38	TTTCATCCTTACAATGATGATACT	38	AAGTACAATGAAAAATTCAAGGGCAAGGCCAAATTGACTGTAGAAAAATCCTCTAGCACAGTCTACTTGGAGCTCAGCCGATTAACATCTGATGACTCTGCTGTTTATTAC	38	TGTGCAAGGTATGGTAACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTTYP	IEWMKQNHGKSLEWIGN	FHPYNDDT	KYNEKFKGKAKLTVEKSSSTVYLELSRLTSDDSAVYY	CARYGNYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:0:219:219:-5:-5:226:226:-3:246:277::
76	401	0.000907714	GGCTACACCTTCACCAGCTCCTGGATGCACTGGGCGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGAGAGATTCATCCTAATAGTGGTAATACTAACTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGTAGACACATCCTCCAGCACAGCCTACGTGGATCTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTACTGTGCAAGCGGCTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-64*00(2215.2)	nan	IGHJ2*00(470)	IGHM*00(64.4)	232\|450\|471\|0\|218\|ST240CSA251CST266CSA304GST305ASG326ASA352GSA377CSA397GSC400GSA402T\|1861.0	nan	21\|68\|68\|221\|268\|\|470.0	nan	nan	nan	GGCTACACCTTCACCAGCTCCTGG	38	ATGCACTGGGCGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGAGAG	38	ATTCATCCTAATAGTGGTAATACT	38	AACTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGTAGACACATCCTCCAGCACAGCCTACGTGGATCTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTAC	38	TGTGCAAGCGGCTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTSSW	MHWAKQRPGQGLEWIGE	IHPNSGNT	NYNEKFKGKATLTVDTSSSTAYVDLSSLTSEDSAVYY	CASGYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:-1:218:::::221:-1:237:268::
77	393	0.000889605	GGCTACACCTTTACCAGCTACTGGATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGCGCTATTTATCCTGGAAATAGTGATACTAGCTACAACCAGAAGTTCAAGGGCAAGGCCAAACTGACTGCAGTCACATCCACCAGCACTGCCTACATGGAGCTCAGCAGCCTGACAAATGAGGACTCTGCGGTCTATTACTGTACAAGAGAGGTACGAAACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-5*00(2567.2)	IGHD5-1*00(30)	IGHJ2*00(360)	IGHG1*00(35.5)	232\|451\|471\|0\|219\|SA240CSA300TSG303CSG382A\|2074.0	2\|8\|30\|220\|226\|\|30.0	32\|68\|68\|229\|265\|\|360.0	nan	nan	nan	GGCTACACCTTTACCAGCTACTGG	38	ATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGCGCT	38	ATTTATCCTGGAAATAGTGATACT	38	AGCTACAACCAGAAGTTCAAGGGCAAGGCCAAACTGACTGCAGTCACATCCACCAGCACTGCCTACATGGAGCTCAGCAGCCTGACAAATGAGGACTCTGCGGTCTATTAC	38	TGTACAAGAGAGGTACGAAACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTSYW	MHWVKQRPGQGLEWIGA	IYPGNSDT	SYNQKFKGKAKLTAVTSTSTAYMELSSLTNEDSAVYY	CTREVRNW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:220:8:-12:226:229:-12:234:265::
78	393	0.000889605	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACAATGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATCGAGGGGTAGTAGCTATTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1480.9)	IGHD1-1*00(45)	IGHJ4*00(550)	IGHM*00(64.9)	232\|453\|471\|0\|221\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1311.0	34\|43\|69\|225\|234\|\|45.0	19\|74\|74\|234\|289\|\|550.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACAATGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATCGAGGGGTAGTAGCTATTACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYNGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARSRGSSYYYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:2:221:225:-11:-3:234:234:1:258:289::
79	390	0.000882814	GGGTTTTCATTAACCAGCTATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTAATATGGGCTGGTGGAAGCACAAATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTACTGTGCCAGAGATTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-9*00(2579.7)	IGHD1-1*00(55)	IGHJ1*00(551)	IGHM*00(65.2)	232\|446\|469\|0\|214\|SG259CSG314CSA339G\|2053.0	26\|37\|69\|217\|228\|\|55.0	15\|73\|73\|228\|286\|SA45G\|551.0	nan	nan	nan	GGGTTTTCATTAACCAGCTATGGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTA	38	ATATGGGCTGGTGGAAGCACA	38	AATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGAGATTACTACGGTAGTAGCTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQPPGKGLEWLGV	IWAGGST	NYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CARDYYGSSYWYFDVW	GAGTTVTVSS_	:::::0:24:75:96:207:-3:214:217:-3:-9:228:228:5:255:286::
80	385	0.000871496	GGCTTCAACATTAAAGACACCTATATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGGATTGATCCTGCGAATGGTAATACTAAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTACTGTGCTCGGATCTATGATGGTTACTACGGCTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV14-3*00(2512.3)	IGHD2-3*00(100)	IGHJ2*00(380)	IGHM*00(65)	232\|448\|471\|0\|216\|SA247GSC338AST369ASA433G\|2044.0	15\|35\|51\|218\|238\|\|100.0	30\|68\|68\|239\|277\|\|380.0	nan	nan	nan	GGCTTCAACATTAAAGACACCTAT	38	ATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGG	38	ATTGATCCTGCGAATGGTAATACT	38	AAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTAC	38	TGTGCTCGGATCTATGATGGTTACTACGGCTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFNIKDTY	MHWVKQRPEQGLEWIGR	IDPANGNT	KYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYY	CARIYDGYYGYW	GQGTTLTVSS_	:::::0:24:75:99:210:-3:216:218:2:1:238:239:-10:246:277::
81	378	0.000855651	GGCTACACCTTTACTAGCTACTGGATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGATACATTAATCCTAGCACTGGTTATACTGAGTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTGCAGACAAATCCTCCAGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATTACTGTGCAAGATACTGGTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-700(2577.5),IGHV1-400(2535.2)	IGHD4-1*00(25)	IGHJ2*00(460)	IGHM*00(64.9)	232\|452\|471\|0\|220\|SG320CSA331GSG402ASA419C\|2084.0;232\|452\|471\|0\|220\|SA253TSC254GSG320CSA331G\|2084.0	14\|19\|33\|220\|225\|\|25.0	22\|68\|68\|225\|271\|\|460.0	nan	nan	nan	GGCTACACCTTTACTAGCTACTGG	38	ATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGATAC	38	ATTAATCCTAGCACTGGTTATACT	38	GAGTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTGCAGACAAATCCTCCAGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATTAC	38	TGTGCAAGATACTGGTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTSYW	MHWVKQRPGQGLEWIGY	INPSTGYT	EYNQKFKDKATLTADKSSSTAYMQLSSLTSEDSAVYY	CARYWYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:220:-3:-3:225:225:-2:240:271::
82	375	0.00084886	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACAATGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATATGATTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1466.2)	nan	IGHJ4*00(502)	IGHM*00(64.9)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1301.0	nan	18\|74\|74\|218\|274\|ST22GSC24T\|502.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACAATGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATATGATTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYNGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARYDYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:1:220:::::218:2:243:274::
83	371	0.000839805	GGATTCACTTTCAGTAGCTATGGCATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACCATTAGTAGTGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGACAGTATGGTAACTACTATGGTAACTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-6*00(2714.2)	IGHD2-8*00(60)	IGHJ2*00(480)	IGHM*00(64.7)	232\|451\|471\|0\|219\|\|2190.0	22\|34\|51\|222\|234\|\|60.0	20\|68\|68\|241\|289\|\|480.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATGGC	38	ATGTCTTGGGTTCGCCAGACTCCAGACAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTAGTGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGACAGTATGGTAACTACTATGGTAACTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFTFSSYG	MSWVRQTPDKRLEWVAT	ISSGGSYT	YYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CARQYGNYYGNYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:222:-5:0:234:241:0:258:289::
84	369	0.000835278	GGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCGGAAAAGAGGCTGGAGTGGGTCGCAACCATTAGTGATGGTGGTAGTTACACCTACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACAACCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTACTGTGCAAGATATAGGTACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-400(2436.5),IGHV5-600(2408.6)	nan	IGHJ4*00(520)	IGHM*00(64.9)	232\|452\|471\|0\|220\|SA247GSG248ASG253TSC254ASC260ASA344GSA348GSC354GSC409ASA410G\|1910.0;232\|452\|471\|0\|220\|SA247GSG248ASG253TSG254ASC260ASA279GSC282ASA313GSG314ASC389A\|1910.0	nan	22\|74\|74\|225\|277\|\|520.0	nan	nan	nan	GGATTCACTTTCAGTGACTATTAC	38	ATGTATTGGGTTCGCCAGACTCCGGAAAAGAGGCTGGAGTGGGTCGCAACC	38	ATTAGTGATGGTGGTAGTTACACC	38	TACTATCCAGACAGTGTGAAGGGGCGATTCACCATCTCCAGAGACAATGCCAAGAACAACCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACAGCCATGTATTAC	38	TGTGCAAGATATAGGTACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GFTFSDYY	MYWVRQTPEKRLEWVAT	ISDGGSYT	YYPDSVKGRFTISRDNAKNNLYLQMSSLKSEDTAMYY	CARYRYYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:1:220:::::225:-2:246:277::
85	365	0.000826224	GGATACATCTTCACCAGCTACTGGATTCACTGGGTAAAACAGAGGTCTGGACAGGGCCTTGAGTGGATTGCAAGGATTTATCCTGGAACTGGTAGTACTTACTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACTGCCTACATGCAGCTCAGCAGCCTGAAATCTGAGGACTCTGCTGTCTATTTCTGTGCAAGAGGGAACTATGACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-76*00(2136.9)	nan	IGHJ4*00(521)	IGHM*00(65.2)	232\|451\|471\|0\|219\|SC234ASC239TST240CST246CSG247ASA248GSA254GST255GSA258TSA259CSG267ASG270ASC277TSA288CSG320CSA326GSA375CSC416A\|1668.0	nan	19\|74\|74\|225\|280\|ST22G\|521.0	nan	nan	nan	GGATACATCTTCACCAGCTACTGG	38	ATTCACTGGGTAAAACAGAGGTCTGGACAGGGCCTTGAGTGGATTGCAAGG	38	ATTTATCCTGGAACTGGTAGTACT	38	TACTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTGCAGACAAATCCTCCAGCACTGCCTACATGCAGCTCAGCAGCCTGAAATCTGAGGACTCTGCTGTCTATTTC	38	TGTGCAAGAGGGAACTATGACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYIFTSYW	IHWVKQRSGQGLEWIAR	IYPGTGST	YYNEKFKGKATLTADKSSSTAYMQLSSLKSEDSAVYF	CARGNYDYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:0:219:::::225:1:249:280::
86	363	0.000821696	GGCTACACCTTCACAAGCTATGATATAAACTGGGTGAGGCAGAGGCCTGAACAGGGACTTGAGTGGATTGGATGGATTTTTCCTGGAGATGGTAGTACTAAGTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTACAGACAAATCCTCCAGCACAGCCTACATGCAGCTCAGCAGGCTGACATCTGAGGACTCTGCTGTCTATTTCTGTGCAAGAACTATGATTACTGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-85*00(2197.2)	IGHD2-4*00(40)	IGHJ4*00(470)	IGHM*00(65)	232\|454\|471\|0\|222\|SC252TSA269GSG281ASA311TSA316GST364CSG370AST371CSC377ASG393CSG400CSC406ASA407GSC411GSG432TST451A\|1756.0	23\|31\|51\|222\|230\|\|40.0	27\|74\|74\|230\|277\|\|470.0	nan	nan	nan	GGCTACACCTTCACAAGCTATGAT	38	ATAAACTGGGTGAGGCAGAGGCCTGAACAGGGACTTGAGTGGATTGGATGG	38	ATTTTTCCTGGAGATGGTAGTACT	38	AAGTACAATGAGAAGTTCAAGGGCAAGGCCACACTGACTACAGACAAATCCTCCAGCACAGCCTACATGCAGCTCAGCAGGCTGACATCTGAGGACTCTGCTGTCTATTTC	38	TGTGCAAGAACTATGATTACTGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTSYD	INWVRQRPEQGLEWIGW	IFPGDGST	KYNEKFKGKATLTTDKSSSTAYMQLSRLTSEDSAVYF	CARTMITAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:3:222:222:-6:-3:230:230:-7:246:277::
87	362	0.000819433	GGCTACACCTTCACCAGCTACTGGATTAACTGGATAAAACAGAGGCCTGGACAGGGCCTTGAGTGGATAGGACGTATTGCTCCTGGAAGTGGTAGTACTTACTACAATGAAATGTTCAAGGGCAAGGCAACACTGACTGTAGACACATCCTCCAGCACAGCCTACATTCAGCTCAGCAGCCTGTCATCTGAGGACTCTGCTGTCTATTTCTGTGCGACTATGATTACGACTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-5500(1858.2),IGHV1-7700(1857.9)	IGHD2-4*00(70)	IGHJ2*00(410)	IGHM*00(65.1)	232\|447\|471\|0\|215\|SA258TSC260ASG265ASG267ASG270ASA285GST300ASG304CSA305GST310GSA311CST318ASA331TSG342ASA344TSA352GSC360ASG399TSA415TSG432TSA440T\|1541.0;232\|447\|471\|0\|215\|ST246CSG247ASA248GSA254GST255GSA258TSG265ASG267ASG270AST300ASA304CSA305GSG306TSG311CSG342ASA344TSC360ASC371TSA377CSG399TSA415TSA432T\|1512.0	20\|34\|51\|216\|230\|\|70.0	27\|68\|68\|230\|271\|\|410.0	nan	nan	nan	GGCTACACCTTCACCAGCTACTGG	38	ATTAACTGGATAAAACAGAGGCCTGGACAGGGCCTTGAGTGGATAGGACGT	38	ATTGCTCCTGGAAGTGGTAGTACT	38	TACTACAATGAAATGTTCAAGGGCAAGGCAACACTGACTGTAGACACATCCTCCAGCACAGCCTACATTCAGCTCAGCAGCCTGTCATCTGAGGACTCTGCTGTCTATTTC	38	TGTGCGACTATGATTACGACTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTSYW	INWIKQRPGQGLEWIGR	IAPGSGST	YYNEMFKGKATLTVDTSSSTAYIQLSSLSSEDSAVYF	CATMITTDYW	GQGTTLTVSS_	:::::0:24:75:99:210:-4:215:216:-3:0:230:230:-7:240:271::
88	353	0.00079906	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTATGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATACTATGGTTACGACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1471.9)	IGHD2-7*00(60)	IGHJ4*00(510)	IGHM*00(65)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1272.0	20\|32\|51\|220\|232\|\|60.0	23\|74\|74\|232\|283\|\|510.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTATGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATACTATGGTTACGACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYYGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARYYGYDYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:1:220:220:-3:-2:232:232:-3:252:283::
89	351	0.000794533	GGATTCACTTTCAGTAGCTATACCATGTCTTGGGTTCGCCAGACTCCAGAGAAGAGGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTACTATCCAGACACTGTAAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACGGCCATGTATTACTGTACCTACGGCTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV5-12*00(2397.4)	IGHD5-100(35),IGHD5-200(35),IGHD5-3*00(35)	IGHJ4*00(500)	IGHM*00(64.8)	232\|445\|471\|0\|213\|SG247ASA248GST253ASA254CSA260CST333CSC409ASA429G\|1898.0	14\|21\|30\|213\|220\|\|35.0;14\|21\|30\|213\|220\|\|35.0;14\|21\|30\|213\|220\|\|35.0	24\|74\|74\|221\|271\|\|500.0	nan	nan	nan	GGATTCACTTTCAGTAGCTATACC	38	ATGTCTTGGGTTCGCCAGACTCCAGAGAAGAGGCTGGAGTGGGTCGCATAC	38	ATTAGTAATGGTGGTGGTAGCACC	38	TACTATCCAGACACTGTAAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCAGTCTGAAGTCTGAGGACACGGCCATGTATTAC	38	TGTACCTACGGCTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GFTFSSYT	MSWVRQTPEKRLEWVAY	ISNGGGST	YYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYY	CTYGYAMDYW	GQGTSVTVSS_	:::::0:24:75:99:210:-6:213:213:-4:1:220:221:-4:240:271::
90	350	0.000792269	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGATTGAGGGGTTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1461.7)	nan	IGHJ2*00(370)	IGHM*00(65)	232\|452\|471\|0\|220\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1243.0	nan	31\|68\|68\|228\|265\|\|370.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGATTGAGGGGTTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARLRGYW	GQGTTLTVSS_	:::::0:24:75:99:210:1:220:::::228:-11:234:265::
91	346	0.000783215	GGGTTCTCATTAACCAGCTATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTCCAAACTGATGACACAGCCATGTACTACTGTGCCAGACAAATCTATGATGGCTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-6*00(2660)	IGHD2-3*00(50)	IGHJ1*00(511)	IGHM*00(64.9)	232\|448\|468\|0\|216\|\|2160.0	16\|26\|51\|219\|229\|\|50.0	19\|73\|73\|229\|283\|SA45G\|511.0	nan	nan	nan	GGGTTCTCATTAACCAGCTATGGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTG	38	ATATGGAGTGATGGAAGCACA	38	ACCTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTCCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGACAAATCTATGATGGCTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQPPGKGLEWLVV	IWSDGST	TYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CARQIYDGYWYFDVW	GAGTTVTVSS_	:::::0:24:75:96:207:0:216:219:1:-8:229:229:1:252:283::
92	344	0.000778688	GGGTTCTCATTAACCGGCTATGGTGTAAACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAATGATATGGGGTGATGGAAGCACAGACTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCAGGTACTACTGTGCCAGACCAGCCTACTATGGTAACTACGACTATGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-600(2426.1),IGHV2-6-800(2358.3)	IGHD2-800(85),IGHD2-500(81),IGHD2-6*00(81)	IGHJ4*00(510)	IGHG2C*00(64)	232\|448\|468\|0\|216\|SA247GSC259AST302GSG304ASA313GSA328GSC329ASC411GST431G\|1899.0;232\|447\|470\|0\|215\|SA247GSG259AST274CSG304ASA306GST317ASA328GST330CST431G\|1889.0	18\|35\|51\|221\|238\|\|85.0;16\|35\|51\|219\|238\|SA25G\|81.0;16\|35\|51\|219\|238\|SA25G\|81.0	23\|74\|74\|238\|289\|\|510.0	nan	nan	nan	GGGTTCTCATTAACCGGCTATGGT	38	GTAAACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAATG	38	ATATGGGGTGATGGAAGCACA	38	GACTATAATTCAGCTCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCAGGTACTAC	38	TGTGCCAGACCAGCCTACTATGGTAACTACGACTATGCTATGGACTACTGG	38	GGTCAAGGAACCTCAGTCACCGTCTCCTCAG	38	nan	GFSLTGYG	VNWVRQPPGKGLEWLGM	IWGDGST	DYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTARYY	CARPAYYGNYDYAMDYW	GQGTSVTVSS_	:::::0:24:75:96:207:0:216:2211:238:238:-3:258:289::
93	342	0.00077416	GGGTTTTCATTAACCAGCTATGGTGTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTAATATGGGCTGGTGGAAGCACAAATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTACTGTGCCAGGTCCTATTACTACGGTAGTAGCTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV2-9*00(2581.1)	IGHD1-1*00(85)	IGHJ2*00(440)	IGHM*00(64.7)	232\|446\|469\|0\|214\|SG259CSG314CSA339G\|2053.0	25\|42\|69\|219\|236\|\|85.0	24\|68\|68\|236\|280\|\|440.0	nan	nan	nan	GGGTTTTCATTAACCAGCTATGGT	38	GTACACTGGGTTCGCCAGCCTCCAGGAAAGGGTCTGGAGTGGCTGGGAGTA	38	ATATGGGCTGGTGGAAGCACA	38	AATTATAATTCGGCTCTCATGTCCAGACTGAGCATCAGCAAAGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTGCAAACTGATGACACAGCCATGTACTAC	38	TGTGCCAGGTCCTATTACTACGGTAGTAGCTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GFSLTSYG	VHWVRQPPGKGLEWLGV	IWAGGST	NYNSALMSRLSISKDNSKSQVFLKMNSLQTDDTAMYY	CARSYYYGSSFDYW	GQGTTLTVSS_	:::::0:24:75:96:207:-3:214:219:-2:-4:236:236:-4:249:280::
94	341	0.000771897	GGCTACACCTTTACTAGCTACTGGATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGATACATTAATCCTAGCACTGGTTATACTGAGTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTGCAGACAAATCCTCCAGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATTACTGTGCAAGGCCCGGATGGTTACTACACTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-700(2562),IGHV1-400(2533.4)	IGHD2-3*00(60)	IGHJ1*00(501)	IGHM*00(64.9)	232\|450\|471\|0\|218\|SG320CSA331GSG402ASA419C\|2064.0;232\|450\|471\|0\|218\|SA253TSC254GSG320CSA331G\|2064.0	22\|34\|51\|223\|235\|\|60.0	20\|73\|73\|236\|289\|SA45G\|501.0	nan	nan	nan	GGCTACACCTTTACTAGCTACTGG	38	ATGCACTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGATAC	38	ATTAATCCTAGCACTGGTTATACT	38	GAGTACAATCAGAAGTTCAAGGACAAGGCCACATTGACTGCAGACAAATCCTCCAGCACAGCCTACATGCAACTGAGCAGCCTGACATCTGAGGACTCTGCAGTCTATTAC	38	TGTGCAAGGCCCGGATGGTTACTACACTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GYTFTSYW	MHWVKQRPGQGLEWIGY	INPSTGYT	EYNQKFKDKATLTADKSSSTAYMQLSSLTSEDSAVYY	CARPGWLLHYWYFDVW	GAGTTVTVSS_	:::::0:24:75:99:210:-1:218:223:-5:0:235:236:0:258:289::
95	337	0.000762842	GGCTACACATTCACTGATTATGCTATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTTATTAGTACTTACTCTGGTAATACAAACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTACTGTGCAAGACAGCTCGGCTACTTTGACTACTGGGGCCAAGGCACCACTCTCACAGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-19*00(1387.1)	IGHD3-1*00(40)	IGHJ2*00(470)	IGHM*00(64.6)	232\|451\|471\|0\|219\|SA234CSC249TSC252TST253GSA254CSA259CSC276TSG281CSC288TST291ASA311GSC313ASA319TSA320CSC321TSG325ASG326AST330ASG332ASC348TST364AST372ASG378ASC396TSG402ASC405TSA406GSA407CSC411ASC412TSC426TSA432CSG433A\|1233.0	21\|29\|51\|219\|227\|\|40.0	21\|68\|68\|227\|274\|\|470.0	nan	nan	nan	GGCTACACATTCACTGATTATGCT	38	ATGCACTGGGTGAAGCAGAGTCATGCAAAGAGTCTAGAGTGGATTGGAGTT	38	ATTAGTACTTACTCTGGTAATACA	38	AACTACAACCAGAAGTTTAAGGGCAAGGCCACAATGACTGTAGACAAATCCTCCAGCACAGCCTATATGGAACTTGCCAGATTGACATCTGAGGATTCTGCCATCTATTAC	38	TGTGCAAGACAGCTCGGCTACTTTGACTACTGG	38	GGCCAAGGCACCACTCTCACAGTCTCCTCAG	38	nan	GYTFTDYA	MHWVKQSHAKSLEWIGV	ISTYSGNT	NYNQKFKGKATMTVDKSSSTAYMELARLTSEDSAIYY	CARQLGYFDYW	GQGTTLTVSS_	:::::0:24:75:99:210:0:219:219:-4:-5:227:227243:274::
96	333	0.000753788	GGCTACTCCTTCACCAGCTACTGGATGAACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGCATGATTCATCCTTCCGATAGTGAAACTAGGTTAAATCAGAAGTTCAAGGACAAGGCCACATTGACTGTAGACAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCCCGACATCTGAGGACTCTGCGGTCTATTACTGTGCAAGAACTGGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-7400(2147),IGHV1-6100(2089.9)	IGHD4-1*00(25)	IGHJ3*00(450)	IGHM*00(64.7)	232\|449\|471\|0\|217\|SA238TSC259ASC282ASA303CSG305TST318CST327ASA332GSC333GSA335TSC336ASA342GSG353ASG402AST413C\|1735.0;232\|454\|471\|0\|222\|SA238TSG259AST261CSA294GST303CSA305TSC306GST310CSC312TST318CSC331ASA332GSC333GSA335TSC336ASA342GSG402AST413CST451A\|1669.0	13\|18\|33\|218\|223\|\|25.0	23\|68\|68\|223\|268\|\|450.0	nan	nan	nan	GGCTACTCCTTCACCAGCTACTGG	38	ATGAACTGGGTGAAGCAGAGGCCTGGACAAGGCCTTGAGTGGATTGGCATG	38	ATTCATCCTTCCGATAGTGAAACT	38	AGGTTAAATCAGAAGTTCAAGGACAAGGCCACATTGACTGTAGACAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCCCGACATCTGAGGACTCTGCGGTCTATTAC	38	TGTGCAAGAACTGGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYSFTSYW	MNWVKQRPGQGLEWIGM	IHPSDSET	RLNQKFKDKATLTVDKSSSTAYMQLSSPTSEDSAVYY	CARTGFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:-2:217:218:-2:-4:223:223:-3:237:268::
97	325	0.000735679	GGCTTCAACATTAAAGACACCTATATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGGATTGATCCTGCGAATGGTAATACTAAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTACTGTGCTGTACCCTGGTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV14-3*00(2514.2)	nan	IGHJ3*00(480)	IGHM*00(64.5)	232\|448\|471\|0\|216\|SA247GSC338AST369ASA433G\|2044.0	nan	20\|68\|68\|220\|268\|\|480.0	nan	nan	nan	GGCTTCAACATTAAAGACACCTAT	38	ATGCACTGGGTGAAGCAGAGGCCTGAACAGGGCCTGGAGTGGATTGGAAGG	38	ATTGATCCTGCGAATGGTAATACT	38	AAATATGACCCGAAGTTCCAGGGCAAGGCCACTATAACAGCAGACACATCCTCCAACACAGCCTACCTGCAGCTCAGCAGCCTGACATCTGAGGACACTGCCGTCTATTAC	38	TGTGCTGTACCCTGGTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GFNIKDTY	MHWVKQRPEQGLEWIGR	IDPANGNT	KYDPKFQGKATITADTSSNTAYLQLSSLTSEDTAVYY	CAVPWFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:-3:216:::::220:0:237:268::
98	325	0.000735679	GGCTACACCTTTACTAGCTACTGGATGCAGTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGGGCTATTTATCCTGGAGATGGTGATACTAGGTACACTCAGAAGTTCAAGGGCAAGGCCACATTGACTGCAGATAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCTTGGCATCTGAGGACTCTGCGGTCTATTACTGTGCAAGATCGGCCTATGGTAACTACTTTGCTTACTGGGGCCAAGGGACTCTGGTCACTGTCTCTGCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV1-400(1986.3),IGHV1-700(1966.4)	IGHD2-8*00(65)	IGHJ3*00(430)	IGHM*00(64.9)	232\|453\|471\|0\|221\|SA253TSC254GSC261GSA303GST304GSA305CSC306TSA310TSA316GSC318ASA319GSG320AST325GSA332GSA338CSA353GSC375TSG405CSC412TSA415GSA432G\|1601.0;232\|453\|471\|0\|221\|SC261GSA303GST304GSA305CSC306TSA310TSA316GSC318ASA319GSG320AST325GSA332GSA338CSA353GSC375TSG402ASG405CSC412TSA415GSA419CSA432G\|1601.0	21\|34\|51\|224\|237\|\|65.0	25\|68\|68\|237\|280\|\|430.0	nan	nan	nan	GGCTACACCTTTACTAGCTACTGG	38	ATGCAGTGGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATTGGGGCT	38	ATTTATCCTGGAGATGGTGATACT	38	AGGTACACTCAGAAGTTCAAGGGCAAGGCCACATTGACTGCAGATAAATCCTCCAGCACAGCCTACATGCAACTCAGCAGCTTGGCATCTGAGGACTCTGCGGTCTATTAC	38	TGTGCAAGATCGGCCTATGGTAACTACTTTGCTTACTGG	38	GGCCAAGGGACTCTGGTCACTGTCTCTGCAG	38	nan	GYTFTSYW	MQWVKQRPGQGLEWIGA	IYPGDGDT	RYTQKFKGKATLTADKSSSTAYMQLSSLASEDSAVYY	CARSAYGNYFAYW	GQGTLVTVSA_	:::::0:24:75:99:210:2:221:224:-4:0:237:237:-5:249:280::
99	325	0.000735679	GGGTTCACCTTCACTGATTACTACATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTTATTAGAAACAAAGCTAATGGTTACACAACAGAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTACTGTGCAAGATCCCCCTACGGCTACTGGTACTTCGATGTCTGGGGCGCAGGGACCACGGTCACCGTCTCCTCAG	GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	IGHV7-3*00(2614.3)	IGHD5-100(30),IGHD5-200(30),IGHD5-3*00(30)	IGHJ1*00(511)	IGHM*00(64.7)	232\|458\|481\|0\|226\|SA234GSG282AST414CSG415A\|2144.0	15\|21\|30\|229\|235\|\|30.0;15\|21\|30\|229\|235\|\|30.0;15\|21\|30\|229\|235\|\|30.0	19\|73\|73\|235\|289\|SA45G\|511.0	nan	nan	nan	GGGTTCACCTTCACTGATTACTAC	38	ATGAGCTGGGTCCGCCAGCCTCCAGGAAAGGCACTTGAGTGGTTGGGTTTT	38	ATTAGAAACAAAGCTAATGGTTACACAACA	38	GAGTACAGTGCATCTGTGAAGGGTCGGTTCACCATCTCCAGAGATAATTCCCAAAGCATCCTCTATCTTCAAATGAACACCCTGAGAGCTGAGGACAGTGCCACTTATTAC	38	TGTGCAAGATCCCCCTACGGCTACTGGTACTTCGATGTCTGG	38	GGCGCAGGGACCACGGTCACCGTCTCCTCAG	38	nan	GFTFTDYY	MSWVRQPPGKALEWLGF	IRNKANGYTT	EYSASVKGRFTISRDNSQSILYLQMNTLRAEDSATYY	CARSPYGYWYFDVW	GAGTTVTVSS_	:::::0:24:75:105:216:-3:226:229:-5:1:235:235:1:258:289::

Now, since we have multiple files ist easier to process them all together instead of running the same command multiple times. One of the ways to achieve it is to use GNU Parallel:

#!/usr/bin/env bash

mkdir -p results/

ls raw/*_1* | \
    parallel -j 4 \
    'mixcr analyze generic-amplicon -f \
    --species mmu \
    --rna \
    --rigid-left-alignment-boundary \
    --rigid-right-alignment-boundary \
    --tagPattern "^N{"4"}ccctcctttaattccc{"21"}(R1:*)\^N{"4"}gaggagagagagagagN{"26"}(R2:*)" \
    --assemble-clonotypes-by {CDR1Begin:FR4End} \
    {} \
    {=s:R1:R2:=} \
    {=s:.*/:results/:;s:_R.*::=}'

Under the hood

Under the hood, the command from above actually executes the following pipeline of MiXCR actions:

`align`

Performs:

alignment of raw sequencing reads against reference database of V-, D-, J- and C- gene segments
pattern matching of tag pattern sequence and extraction of barcodes

mixcr align \
    -p generic-amplicon \
    --species mmu \
    --tag-pattern '^(R1:*)\^(UMI:N{12})' \
    --report result/M1_4T1_replica1_Blood.align.report.txt \
    --json-report result/M1_4T1_replica1_Blood.align.report.json \
    -OvParameters.geneFeatureToAlign="VTranscriptWithout5UTRWithP" \
    -OvParameters.parameters.floatingLeftBound=false \
    -OjParameters.parameters.floatingRightBound=false \
    -OcParameters.parameters.floatingRightBound=false \
    raw/M1_4T1_replica1_Blood_R1.fastq.gz \
    raw/M1_4T1_replica1_Blood_R2.fastq.gz \
    results/M1_4T1_replica1_Blood.vdjca

Options --report and --json-report are specified here explicitly.

This step utilizes all available CPUs and scales perfectly. When there are a lot of CPUs, the only limiting factor is the speed of disk I/O. To limit the number of used CPUs one can pass --threads N option.

`assemble`

Assembles clonotypes and applies several layers of errors correction:

assembly consensus CDR3 sequence
quality-awared correction for sequencing errors
clustering to correct for PCR errors

mixcr assemble \
    --report results/M1_4T1_replica1_Blood.assemble.report.txt \
    --json-report results/P15-T0-TIGIT.assemble.report..json \
    -OassemblingFeatures={CDR1Begin:FR4End} \
    results/M1_4T1_replica1_Blood.vdjca \
    results/M1_4T1_replica1_Blood.clns

Options --report and --json-report are specified here explicitly so that the report files will be appended with assembly report.

`exportClones`

Finally, to export clonotype tables in tabular form exportClones is used:

mixcr exportClones \
     --chains IGH \
     results/M1_4T1_replica1_Blood.clns \
     results/M1_4T1_replica1_Blood.clones.tsv

Quality control

Now when the upstream analysis is finished we can move on to quality control. First lets look at the alignment report plot.

mixcr exportQc align results/*.clns figs/alignQc.pdf

We see that all samples have a very high score of successfully aligned reads. No significant issues present.

Next, lets look at the chain usage distribution.

mixcr exportQc chainUsage results/*.clns figs/chainUsage.pdf

We don't see any contamination from other Ig chains. As expected, all samples consist only of IGH chains.

Downstream analysis

There are two types of basic downstream analysis: individual and overlap. Individual computes CDR3 metrics, diversity and gene usage metrics for each dataset. Overlap computes statistical metrics of repertoire overlap. In both cases MiXCR applies appropriate sample normalization.

To run postanalysis routines we need to prepare a metadata file in a .tsv or .csv form. Metadata must contain a sample column which will be used to match metadata with cloneset files. Bellow you can find a metadata table for our samples.

Metadata

sample	mice_id	condition	tissue	replica
M1_4T1_Blood_S2	M1	4T1	Blood	1
M1_4T1_Blood_S6	M1	4T1	Blood	2
M1_4T1_BM_S4	M1	4T1	BM	1
M1_4T1_BM_S8	M1	4T1	BM	2
M1_4T1_DLN_S3	M1	4T1	DLN	1
M1_4T1_DLN_S7	M1	4T1	DLN	2
M1_4T1_Tumor_S5	M1	4T1	Tumor	1
M1_4T1_Tumor_S1	M1	4T1	Tumor	2
M2_4T1_Blood_S2	M2	4T1	Blood	1
M2_4T1_Blood_S6	M2	4T1	Blood	2
M2_4T1_BM_S4	M2	4T1	BM	1
M2_4T1_BM_S8	M2	4T1	BM	2
M2_4T1_DLN_S3	M2	4T1	DLN	1
M2_4T1_DLN_S7	M2	4T1	DLN	2
M2_4T1_Tumor_S1	M2	4T1	Tumor	1
M2_4T1_Tumor_S5	M2	4T1	Tumor	2
M3_4T1_Blood_S10	M3	4T1	Blood	1
M3_4T1_Blood_S9	M3	4T1	Blood	2
M3_4T1_BM_S7	M3	4T1	BM	1
M3_4T1_BM_S8	M3	4T1	BM	2
M3_4T1_DLN_S11	M3	4T1	DLN	1
M3_4T1_DLN_S12	M3	4T1	DLN	2
M3_4T1_Tumor_S13	M3	4T1	Tumor	1
M3_4T1_Tumor_S14	M3	4T1	Tumor	2
M4_4T1_Blood_S2	M4	4T1	Blood	1
M4_4T1_Blood_S7	M4	4T1	Blood	2
M4_4T1_BM_S10	M4	4T1	BM	1
M4_4T1_BM_S5	M4	4T1	BM	2
M4_4T1_DLN_S4	M4	4T1	DLN	1
M4_4T1_DLN_S9	M4	4T1	DLN	2
M4_4T1_Tumor_S1	M4	4T1	Tumor	1
M4_4T1_Tumor_S6	M4	4T1	Tumor	2
N1_control_Blood_S5	N1	control	Blood	1
N1_control_Blood_S6	N1	control	Blood	2
N1_control_BM_S1	N1	control	BM	1
N1_control_BM_S2	N1	control	BM	2
N1_control_LN_S3	N1	control	LN	1
N1_control_LN_S4	N1	control	LN	2
N2_control_Blood_S5	N2	control	Blood	1
N2_control_Blood_S6	N2	control	Blood	2
N2_control_BM_S1	N2	control	BM	1
N2_control_BM_S2	N2	control	BM	2
N2_control_LN_S3	N2	control	LN	1
N2_control_LN_S4	N2	control	LN	2

Individual postanalysis

To compute a set of individual metrics we run the following command:

mkdir -p pa/
mixcr postanalysis individual \
    --metadata metadata.tsv \
    --default-downsampling count-reads-auto \
    --default-weight-function read \
    --only-productive \
    --chains IGH \
    --tables pa/pa.i.tsv \
    --preproc-tables pa/preproc.i.tsv \
    results/*.clns \
    pa/individual.json.gz

The meaning of specified options is the following:

--metadata: specified metadata file to use
--default-downsampling: downsampling applied to normalize the clonesets. Without appropriate normalization it is not possible to make a statistical comparisons between datasets. In the considered case we normalize data to the same number of reads, and this number is computed automatically based on the number of unique reads in each clone in each dataset. See reference for all downsampling options. Default downsampling may be overridden for individual metrics.
--default-weight-function: defines weight of each clonotype. Set read beacause there are no barcodes in the data.
--only-productive: drop clonotypes with out-of-frame CDR3 sequences or containing stop codons
--tables: path for postanalysis metrics in a tabular form
--preproc-tables: path for tabular summary of the applied downsampling and other samples preprocessing (for example filtering productive clonotypes)
--chains: Since only IGH chain is present in the samples we can specify it directly.

After execution, we will have the following files:

> ls pa/

# gzipped JSON with all results 
individual.json.gz

# summary of applied preprocessors
preproc.i.IGH.tsv

# diversity tables
pa.i.diversity.IGH.tsv

# CDR3 metrics tables & CDR3 properties
pa.i.cdr3metrics.IGH.tsv

# V- / J- / VJ- usage tables
pa.i.vUsage.IGH.tsv
pa.i.JUsage.IGH.tsv
pa.i.VJUsage.IGH.tsv

# Isotype usage table
pa.i.IsotypeUsage.IGH.tsv

#V Spectratype tables
pa.i.VSpectratype.IGH.tsv
pa.i.VSpectratypeMean.IGH.tsv

Preprocessing summary tables (e.g. preproc.i.IGH.tsv) contain detailed information on how downsampling was applied for each metric (the table bellow shows first 10 rows):

characteristic	sample	preprocessor	nElementsBefore	sumWeightBefore	nElementsAfter	sumWeightAfter	preprocessor#1	nElementsBefore#1	sumWeightBefore#1	nElementsAfter#1	sumWeightAfter#1	preprocessor#2	nElementsBefore#2	sumWeightBefore#2	nElementsAfter#2	sumWeightAfter#2	preprocessor#3	nElementsBefore#3	sumWeightBefore#3	nElementsAfter#3	sumWeightAfter#3	preprocessor#4	nElementsBefore#4	sumWeightBefore#4	nElementsAfter#4	sumWeightAfter#4
IsotypeUsage	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550	nan	nan	nan	nan	nan
IsotypeUsage	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550	nan	nan	nan	nan	nan
IsotypeUsage	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550	nan	nan	nan	nan	nan
IsotypeUsage	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550	nan	nan	nan	nan	nan
IsotypeUsage	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550	nan	nan	nan	nan	nan

See full preprocessing summary:

characteristic	sample	preprocessor	nElementsBefore	sumWeightBefore	nElementsAfter	sumWeightAfter	preprocessor#1	nElementsBefore#1	sumWeightBefore#1	nElementsAfter#1	sumWeightAfter#1	preprocessor#2	nElementsBefore#2	sumWeightBefore#2	nElementsAfter#2	sumWeightAfter#2	preprocessor#3	nElementsBefore#3	sumWeightBefore#3	nElementsAfter#3	sumWeightAfter#3	preprocessor#4	nElementsBefore#4	sumWeightBefore#4	nElementsAfter#4	sumWeightAfter#4
IsotypeUsage	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
IsotypeUsage	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
IsotypeUsage	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
IsotypeUsage	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
IsotypeUsage	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
IsotypeUsage	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
IsotypeUsage	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
IsotypeUsage	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
IsotypeUsage	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
IsotypeUsage	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
IsotypeUsage	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
IsotypeUsage	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
IsotypeUsage	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
IsotypeUsage	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
IsotypeUsage	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
IsotypeUsage	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
IsotypeUsage	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
IsotypeUsage	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
IsotypeUsage	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
IsotypeUsage	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
IsotypeUsage	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
IsotypeUsage	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
IsotypeUsage	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
VSegmentUsage	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
VSegmentUsage	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
VSegmentUsage	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
VSegmentUsage	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
VSegmentUsage	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
VSegmentUsage	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
VSegmentUsage	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
VSegmentUsage	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
VSegmentUsage	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
VSegmentUsage	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
VSegmentUsage	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
VSegmentUsage	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
VSegmentUsage	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
VSegmentUsage	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
VSegmentUsage	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
VSegmentUsage	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
VSegmentUsage	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
VSegmentUsage	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
VSegmentUsage	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
VSegmentUsage	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
VSegmentUsage	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
VSegmentUsage	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
VSegmentUsage	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
JSegmentUsage	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
JSegmentUsage	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
JSegmentUsage	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
JSegmentUsage	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
JSegmentUsage	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
JSegmentUsage	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
JSegmentUsage	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
JSegmentUsage	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
JSegmentUsage	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
JSegmentUsage	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
JSegmentUsage	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
JSegmentUsage	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
JSegmentUsage	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
JSegmentUsage	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
JSegmentUsage	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
JSegmentUsage	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
JSegmentUsage	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
JSegmentUsage	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
JSegmentUsage	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
JSegmentUsage	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
JSegmentUsage	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
JSegmentUsage	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
JSegmentUsage	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
Charge	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
Charge	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
Charge	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
Charge	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
Charge	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
Charge	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
Charge	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
Charge	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
Charge	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
Charge	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
Charge	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
Charge	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
Charge	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
Charge	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
Charge	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
Charge	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
Charge	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
Charge	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
Charge	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
Charge	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
Charge	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
Charge	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
Charge	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
Charge	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
Charge	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
Charge	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
Charge	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
Charge	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
Charge	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
Charge	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
Charge	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
Charge	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
Charge	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
Charge	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
Charge	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
Charge	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
Charge	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
Charge	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
Charge	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
Charge	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
Charge	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
Charge	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
Charge	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
Charge	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
Hydrophobicity	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
Hydrophobicity	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
Hydrophobicity	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
Hydrophobicity	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
Hydrophobicity	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
Hydrophobicity	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
Hydrophobicity	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
Hydrophobicity	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
Hydrophobicity	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
Hydrophobicity	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
Hydrophobicity	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
Hydrophobicity	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
Hydrophobicity	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
Hydrophobicity	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
Hydrophobicity	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
Hydrophobicity	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
Hydrophobicity	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
Hydrophobicity	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
Hydrophobicity	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
Hydrophobicity	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
Hydrophobicity	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
Hydrophobicity	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
Hydrophobicity	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
CDR3 spectratype	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
CDR3 spectratype	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
CDR3 spectratype	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
CDR3 spectratype	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
CDR3 spectratype	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
CDR3 spectratype	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
CDR3 spectratype	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
CDR3 spectratype	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
CDR3 spectratype	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
CDR3 spectratype	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
CDR3 spectratype	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
CDR3 spectratype	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
CDR3 spectratype	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
CDR3 spectratype	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
CDR3 spectratype	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
CDR3 spectratype	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
Surface	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
Surface	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
Surface	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
Surface	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
Surface	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
Surface	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
Surface	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
Surface	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
Surface	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
Surface	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
Surface	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
Surface	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
Surface	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
Surface	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
Surface	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
Surface	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
Surface	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
Surface	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
Surface	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
Surface	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
Surface	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
Surface	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
Surface	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
Surface	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
Surface	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
Surface	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
Surface	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
Surface	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
Surface	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
Surface	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
Surface	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
Surface	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
Surface	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
Surface	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
Surface	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
Surface	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
Surface	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
Surface	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
Surface	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
Surface	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
Surface	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
Surface	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
Surface	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
Surface	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
VSpectratypeMean	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
VSpectratypeMean	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
VSpectratypeMean	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
VSpectratypeMean	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
VSpectratypeMean	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
VSpectratypeMean	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
VSpectratypeMean	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
VSpectratypeMean	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
VSpectratypeMean	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
VSpectratypeMean	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
VSpectratypeMean	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
VSpectratypeMean	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
VSpectratypeMean	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
VSpectratypeMean	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
VSpectratypeMean	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
VSpectratypeMean	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
Diversity Efron-Thisted estimate/Shannon-Wiener diversity/Chao1 estimate/Inverse Simpson index/Normalized Shannon-Wiener index/Gini index/Observed diversity	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
Volume	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
Volume	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
Volume	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
Volume	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
Volume	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
Volume	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
Volume	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
Volume	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
Volume	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
Volume	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
Volume	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
Volume	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
Volume	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
Volume	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
Volume	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
Volume	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
Volume	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
Volume	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
Volume	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
Volume	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
Volume	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
Volume	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
Volume	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
Volume	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
Volume	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
Volume	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
Volume	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
Volume	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
Volume	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
Volume	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
Volume	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
Volume	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
Volume	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
Volume	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
Volume	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
Volume	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
Volume	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
Volume	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
Volume	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
Volume	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
Volume	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
Volume	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
Volume	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
Volume	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
Added N, nt	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
Added N, nt	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
Added N, nt	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
Added N, nt	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
Added N, nt	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
Added N, nt	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
Added N, nt	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
Added N, nt	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
Added N, nt	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
Added N, nt	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
Added N, nt	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
Added N, nt	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
Added N, nt	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
Added N, nt	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
Added N, nt	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
Added N, nt	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
Added N, nt	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
Added N, nt	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
Added N, nt	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
Added N, nt	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
Added N, nt	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
Added N, nt	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
Added N, nt	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
CDR3 length, nt	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
CDR3 length, nt	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
CDR3 length, nt	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
CDR3 length, nt	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
CDR3 length, nt	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
CDR3 length, nt	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
CDR3 length, nt	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
CDR3 length, nt	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
CDR3 length, nt	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
CDR3 length, nt	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
CDR3 length, nt	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
CDR3 length, nt	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
CDR3 length, nt	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
CDR3 length, nt	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
CDR3 length, nt	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
CDR3 length, nt	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
VJSegmentUsage	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
VJSegmentUsage	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
VJSegmentUsage	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
VJSegmentUsage	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
VJSegmentUsage	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
VJSegmentUsage	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
VJSegmentUsage	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
VJSegmentUsage	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
VJSegmentUsage	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
VJSegmentUsage	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
VJSegmentUsage	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
VJSegmentUsage	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
VJSegmentUsage	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
VJSegmentUsage	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
VJSegmentUsage	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
VJSegmentUsage	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
VSpectratype	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
VSpectratype	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
VSpectratype	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
VSpectratype	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
VSpectratype	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
VSpectratype	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
VSpectratype	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
VSpectratype	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
VSpectratype	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
VSpectratype	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
VSpectratype	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
VSpectratype	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
VSpectratype	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
VSpectratype	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
VSpectratype	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
VSpectratype	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
VSpectratype	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
VSpectratype	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
VSpectratype	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
VSpectratype	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
VSpectratype	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
VSpectratype	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
VSpectratype	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
NDN length, nt	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
NDN length, nt	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
NDN length, nt	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
NDN length, nt	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
NDN length, nt	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
NDN length, nt	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
NDN length, nt	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
NDN length, nt	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
NDN length, nt	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
NDN length, nt	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
NDN length, nt	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
NDN length, nt	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
NDN length, nt	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
NDN length, nt	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
NDN length, nt	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
NDN length, nt	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
NDN length, nt	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
NDN length, nt	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
NDN length, nt	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
NDN length, nt	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
NDN length, nt	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
NDN length, nt	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
NDN length, nt	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
d50	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	72121	1.12077e+06	1551	62780	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550	Cumulative top 50.0	30741	125550	1551	62780
d50	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	95523	648857	6462	62778	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550	Cumulative top 50.0	45810	125550	6462	62778
d50	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	92761	1.11126e+06	1394	62775	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550	Cumulative top 50.0	33352	125550	1394	62775
d50	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	1910	1.54254e+06	20	63284	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550	Cumulative top 50.0	653	125550	20	63284
d50	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	8848	248064	29	63275	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550	Cumulative top 50.0	6784	125550	29	63275
d50	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	83727	353453	3450	62776	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550	Cumulative top 50.0	43786	125550	3450	62776
d50	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	5703	14611	459	6906	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808	Cumulative top 50.0	5256	13808	459	6906
d50	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	65294	271110	1793	62778	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550	Cumulative top 50.0	38846	125550	1793	62778
d50	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	8799	846111	401	62798	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550	Cumulative top 50.0	5855	125550	401	62798
d50	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	208134	3.32206e+06	192	62818	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550	Cumulative top 50.0	25345	125550	192	62818
d50	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	32902	104257	5809	47944	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884	Cumulative top 50.0	29813	95884	5809	47944
d50	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	112864	309918	8154	62775	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550	Cumulative top 50.0	61183	125550	8154	62775
d50	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	1453	1.54974e+06	23	63107	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550	Cumulative top 50.0	570	125550	23	63107
d50	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	71252	405065	9031	62778	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550	Cumulative top 50.0	44077	125550	9031	62778
d50	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	70091	1.56128e+06	187	62786	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550	Cumulative top 50.0	20264	125550	187	62786
d50	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	109676	3.11773e+06	192	62825	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550	Cumulative top 50.0	21437	125550	192	62825
d50	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	4320	512431	15	64643	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550	Cumulative top 50.0	2964	125550	15	64643
d50	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	56039	328221	2738	62779	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550	Cumulative top 50.0	34706	125550	2738	62779
d50	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	73450	538465	4034	62775	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550	Cumulative top 50.0	36431	125550	4034	62775
d50	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	15079	291618	835	62796	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550	Cumulative top 50.0	11314	125550	835	62796
d50	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	137249	323560	8121	62776	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550	Cumulative top 50.0	65141	125550	8121	62776
d50	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	54707	495753	500	62778	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550	Cumulative top 50.0	26356	125550	500	62778
d50	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	81594	448685	1043	62778	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550	Cumulative top 50.0	34930	125550	1043	62778
d50	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	66527	255193	1843	62778	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550	Cumulative top 50.0	40241	125550	1843	62778
d50	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	7045	638013	377	62775	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550	Cumulative top 50.0	4869	125550	377	62775
d50	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	52671	823950	6124	62778	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550	Cumulative top 50.0	31986	125550	6124	62778
d50	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	71765	466036	8923	62776	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550	Cumulative top 50.0	43718	125550	8923	62776
d50	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	228959	1.08921e+06	8473	62775	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550	Cumulative top 50.0	62650	125550	8473	62775
d50	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	57007	134123	4158	62775	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550	Cumulative top 50.0	52279	125550	4158	62775
d50	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	49922	254254	1502	62776	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550	Cumulative top 50.0	33366	125550	1502	62776
d50	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	325	3531	2	1826	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429	Cumulative top 50.0	303	3429	2	1826
d50	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	2396	1.46567e+06	79	63023	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550	Cumulative top 50.0	1112	125550	79	63023
d50	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	57445	355874	2799	62778	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550	Cumulative top 50.0	34623	125550	2799	62778
d50	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	5747	699641	16	64880	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550	Cumulative top 50.0	3475	125550	16	64880
d50	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	44577	189592	930	62779	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550	Cumulative top 50.0	33012	125550	930	62779
d50	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	14282	797243	29	62873	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550	Cumulative top 50.0	7173	125550	29	62873
d50	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	66165	982689	465	62778	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550	Cumulative top 50.0	24884	125550	465	62778
d50	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	1325	69980	17	34887	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946	Cumulative top 50.0	1176	67946	17	34887
d50	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	81670	969933	1565	62775	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550	Cumulative top 50.0	30804	125550	1565	62775
d50	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	16665	683973	848	62798	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550	Cumulative top 50.0	11082	125550	848	62798
d50	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	120978	291716	6952	62776	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550	Cumulative top 50.0	62326	125550	6952	62776
d50	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	1419	65516	16	31943	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267	Cumulative top 50.0	1248	63267	16	31943
d50	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	2151	1.6681e+06	60	63262	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550	Cumulative top 50.0	676	125550	60	63262
d50	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic \| Cumulative top 50.0	87256	693306	179	62840	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550	Cumulative top 50.0	24175	125550	179	62840
Strength	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
Strength	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
Strength	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
Strength	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
Strength	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
Strength	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
Strength	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
Strength	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
Strength	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
Strength	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
Strength	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
Strength	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
Strength	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
Strength	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
Strength	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
Strength	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
Strength	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
Strength	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
Strength	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
Strength	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
Strength	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
Strength	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
Strength	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
Strength	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
Strength	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
Strength	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
Strength	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
Strength	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
Strength	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
Strength	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
Strength	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
Strength	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
Strength	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
Strength	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
Strength	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
Strength	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
Strength	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
Strength	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
Strength	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
Strength	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
Strength	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
Strength	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
Strength	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
Strength	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	72121	1.12077e+06	30741	125550	Filter IGH chains	72121	1.12077e+06	72121	1.12077e+06	Filter stops in CDR3, OOF in CDR3	72121	1.12077e+06	64734	1.02411e+06	Downsample automatic	64734	1.02411e+06	30741	125550		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	95523	648857	45810	125550	Filter IGH chains	95523	648857	95523	648857	Filter stops in CDR3, OOF in CDR3	95523	648857	86623	601517	Downsample automatic	86623	601517	45810	125550		nan	nan	nan	nan
CDR3 length, aa	N1_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	92761	1.11126e+06	33352	125550	Filter IGH chains	92761	1.11126e+06	92761	1.11126e+06	Filter stops in CDR3, OOF in CDR3	92761	1.11126e+06	83364	1.01962e+06	Downsample automatic	83364	1.01962e+06	33352	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1910	1.54254e+06	653	125550	Filter IGH chains	1910	1.54254e+06	1910	1.54254e+06	Filter stops in CDR3, OOF in CDR3	1910	1.54254e+06	1771	1.32039e+06	Downsample automatic	1771	1.32039e+06	653	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_DLN_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8848	248064	6784	125550	Filter IGH chains	8848	248064	8848	248064	Filter stops in CDR3, OOF in CDR3	8848	248064	8103	240731	Downsample automatic	8103	240731	6784	125550		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	83727	353453	43786	125550	Filter IGH chains	83727	353453	83727	353453	Filter stops in CDR3, OOF in CDR3	83727	353453	76470	331794	Downsample automatic	76470	331794	43786	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5703	14611	5256	13808	Filter IGH chains	5703	14611	5703	14611	Filter stops in CDR3, OOF in CDR3	5703	14611	5256	13808	Downsample automatic	5256	13808	5256	13808		nan	nan	nan	nan
CDR3 length, aa	N2_control_BM_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	65294	271110	38846	125550	Filter IGH chains	65294	271110	65294	271110	Filter stops in CDR3, OOF in CDR3	65294	271110	59555	254772	Downsample automatic	59555	254772	38846	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_DLN_S12.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	8799	846111	5855	125550	Filter IGH chains	8799	846111	8799	846111	Filter stops in CDR3, OOF in CDR3	8799	846111	8124	810198	Downsample automatic	8124	810198	5855	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	208134	3.32206e+06	25345	125550	Filter IGH chains	208134	3.32206e+06	208134	3.32206e+06	Filter stops in CDR3, OOF in CDR3	208134	3.32206e+06	194979	3.23364e+06	Downsample automatic	194979	3.23364e+06	25345	125550		nan	nan	nan	nan
CDR3 length, aa	N1_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	32902	104257	29813	95884	Filter IGH chains	32902	104257	32902	104257	Filter stops in CDR3, OOF in CDR3	32902	104257	29813	95884	Downsample automatic	29813	95884	29813	95884		nan	nan	nan	nan
CDR3 length, aa	N1_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	112864	309918	61183	125550	Filter IGH chains	112864	309918	112864	309918	Filter stops in CDR3, OOF in CDR3	112864	309918	103169	285540	Downsample automatic	103169	285540	61183	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1453	1.54974e+06	570	125550	Filter IGH chains	1453	1.54974e+06	1453	1.54974e+06	Filter stops in CDR3, OOF in CDR3	1453	1.54974e+06	1317	1.37634e+06	Downsample automatic	1317	1.37634e+06	570	125550		nan	nan	nan	nan
CDR3 length, aa	N2_control_LN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71252	405065	44077	125550	Filter IGH chains	71252	405065	71252	405065	Filter stops in CDR3, OOF in CDR3	71252	405065	64152	373080	Downsample automatic	64152	373080	44077	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	70091	1.56128e+06	20264	125550	Filter IGH chains	70091	1.56128e+06	70091	1.56128e+06	Filter stops in CDR3, OOF in CDR3	70091	1.56128e+06	62434	1.48481e+06	Downsample automatic	62434	1.48481e+06	20264	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	109676	3.11773e+06	21437	125550	Filter IGH chains	109676	3.11773e+06	109676	3.11773e+06	Filter stops in CDR3, OOF in CDR3	109676	3.11773e+06	99507	2.97244e+06	Downsample automatic	99507	2.97244e+06	21437	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_Tumor_S14.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	4320	512431	2964	125550	Filter IGH chains	4320	512431	4320	512431	Filter stops in CDR3, OOF in CDR3	4320	512431	3906	461283	Downsample automatic	3906	461283	2964	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_Blood_S9.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	56039	328221	34706	125550	Filter IGH chains	56039	328221	56039	328221	Filter stops in CDR3, OOF in CDR3	56039	328221	51004	308539	Downsample automatic	51004	308539	34706	125550		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_DLN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	73450	538465	36431	125550	Filter IGH chains	73450	538465	73450	538465	Filter stops in CDR3, OOF in CDR3	73450	538465	66967	499321	Downsample automatic	66967	499321	36431	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_Blood_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	15079	291618	11314	125550	Filter IGH chains	15079	291618	15079	291618	Filter stops in CDR3, OOF in CDR3	15079	291618	13478	273168	Downsample automatic	13478	273168	11314	125550		nan	nan	nan	nan
CDR3 length, aa	N2_control_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	137249	323560	65141	125550	Filter IGH chains	137249	323560	137249	323560	Filter stops in CDR3, OOF in CDR3	137249	323560	126369	307798	Downsample automatic	126369	307798	65141	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_BM_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	54707	495753	26356	125550	Filter IGH chains	54707	495753	54707	495753	Filter stops in CDR3, OOF in CDR3	54707	495753	50774	478430	Downsample automatic	50774	478430	26356	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81594	448685	34930	125550	Filter IGH chains	81594	448685	81594	448685	Filter stops in CDR3, OOF in CDR3	81594	448685	75154	426604	Downsample automatic	75154	426604	34930	125550		nan	nan	nan	nan
CDR3 length, aa	N2_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66527	255193	40241	125550	Filter IGH chains	66527	255193	66527	255193	Filter stops in CDR3, OOF in CDR3	66527	255193	60693	239781	Downsample automatic	60693	239781	40241	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_DLN_S11.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	7045	638013	4869	125550	Filter IGH chains	7045	638013	7045	638013	Filter stops in CDR3, OOF in CDR3	7045	638013	6550	613148	Downsample automatic	6550	613148	4869	125550		nan	nan	nan	nan
CDR3 length, aa	N1_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	52671	823950	31986	125550	Filter IGH chains	52671	823950	52671	823950	Filter stops in CDR3, OOF in CDR3	52671	823950	47041	754429	Downsample automatic	47041	754429	31986	125550		nan	nan	nan	nan
CDR3 length, aa	N2_control_LN_S3.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	71765	466036	43718	125550	Filter IGH chains	71765	466036	71765	466036	Filter stops in CDR3, OOF in CDR3	71765	466036	64445	428849	Downsample automatic	64445	428849	43718	125550		nan	nan	nan	nan
CDR3 length, aa	N1_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	228959	1.08921e+06	62650	125550	Filter IGH chains	228959	1.08921e+06	228959	1.08921e+06	Filter stops in CDR3, OOF in CDR3	228959	1.08921e+06	205257	1.00294e+06	Downsample automatic	205257	1.00294e+06	62650	125550		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57007	134123	52279	125550	Filter IGH chains	57007	134123	57007	134123	Filter stops in CDR3, OOF in CDR3	57007	134123	52279	125550	Downsample automatic	52279	125550	52279	125550		nan	nan	nan	nan
CDR3 length, aa	N1_control_BM_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	49922	254254	33366	125550	Filter IGH chains	49922	254254	49922	254254	Filter stops in CDR3, OOF in CDR3	49922	254254	45458	234149	Downsample automatic	45458	234149	33366	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_Tumor_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	325	3531	303	3429	Filter IGH chains	325	3531	325	3531	Filter stops in CDR3, OOF in CDR3	325	3531	303	3429	Downsample automatic	303	3429	303	3429		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_Tumor_S1.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2396	1.46567e+06	1112	125550	Filter IGH chains	2396	1.46567e+06	2396	1.46567e+06	Filter stops in CDR3, OOF in CDR3	2396	1.46567e+06	2118	744281	Downsample automatic	2118	744281	1112	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_Blood_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	57445	355874	34623	125550	Filter IGH chains	57445	355874	57445	355874	Filter stops in CDR3, OOF in CDR3	57445	355874	52337	335414	Downsample automatic	52337	335414	34623	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_Tumor_S13.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	5747	699641	3475	125550	Filter IGH chains	5747	699641	5747	699641	Filter stops in CDR3, OOF in CDR3	5747	699641	5303	630141	Downsample automatic	5303	630141	3475	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_Blood_S6.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	44577	189592	33012	125550	Filter IGH chains	44577	189592	44577	189592	Filter stops in CDR3, OOF in CDR3	44577	189592	41177	180061	Downsample automatic	41177	180061	33012	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_DLN_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	14282	797243	7173	125550	Filter IGH chains	14282	797243	14282	797243	Filter stops in CDR3, OOF in CDR3	14282	797243	13288	771844	Downsample automatic	13288	771844	7173	125550		nan	nan	nan	nan
CDR3 length, aa	M3_4T1_BM_S8.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	66165	982689	24884	125550	Filter IGH chains	66165	982689	66165	982689	Filter stops in CDR3, OOF in CDR3	66165	982689	61299	948117	Downsample automatic	61299	948117	24884	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_BM_S10.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1325	69980	1176	67946	Filter IGH chains	1325	69980	1325	69980	Filter stops in CDR3, OOF in CDR3	1325	69980	1176	67946	Downsample automatic	1176	67946	1176	67946		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_BM_S4.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	81670	969933	30804	125550	Filter IGH chains	81670	969933	81670	969933	Filter stops in CDR3, OOF in CDR3	81670	969933	74689	885711	Downsample automatic	74689	885711	30804	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_Blood_S2.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	16665	683973	11082	125550	Filter IGH chains	16665	683973	16665	683973	Filter stops in CDR3, OOF in CDR3	16665	683973	14910	642701	Downsample automatic	14910	642701	11082	125550		nan	nan	nan	nan
CDR3 length, aa	N2_control_Blood_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	120978	291716	62326	125550	Filter IGH chains	120978	291716	120978	291716	Filter stops in CDR3, OOF in CDR3	120978	291716	111287	277661	Downsample automatic	111287	277661	62326	125550		nan	nan	nan	nan
CDR3 length, aa	M4_4T1_BM_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	1419	65516	1248	63267	Filter IGH chains	1419	65516	1419	65516	Filter stops in CDR3, OOF in CDR3	1419	65516	1248	63267	Downsample automatic	1248	63267	1248	63267		nan	nan	nan	nan
CDR3 length, aa	M2_4T1_Tumor_S5.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	2151	1.6681e+06	676	125550	Filter IGH chains	2151	1.6681e+06	2151	1.6681e+06	Filter stops in CDR3, OOF in CDR3	2151	1.6681e+06	1922	1.1274e+06	Downsample automatic	1922	1.1274e+06	676	125550		nan	nan	nan	nan
CDR3 length, aa	M1_4T1_DLN_S7.clns	Filter IGH chains \| Filter stops in CDR3, OOF in CDR3 \| Downsample automatic	87256	693306	24175	125550	Filter IGH chains	87256	693306	87256	693306	Filter stops in CDR3, OOF in CDR3	87256	693306	81881	674356	Downsample automatic	81881	674356	24175	125550		nan	nan	nan	nan

Columns explained:

characteristic

metrics name (ex.IsotypeUsage)

sample

name of the .clns file (ex. M2_4T1_BM_S8.clns)

preprocessor

the name of the overall preprocessors chain applied to the dataset. FilterIGHchains|FilterstopsinCDR3,OOFinCDR3|Downsampleautomatic means the data has been subjected to three consecutive preprocessors:

Only IGH chains were filtered
Non-functional clonotypes containing stop-codons and OOF clones were filltered out and only functional were left
Automatic Downsampling function has been applied to normalize sample sizes

nElementsBefore

number of clonotypes before any preprocessing applied (that is in the initial dataset) (ex. 72121 clones)

sumWeightBefore

total weight of all clonotypes before any preprocessing applied. The weight may be either number of reads, UMIs or cells, depending on the selected downsampling. In this case it represents the total number of reads that contribute to clonotypes (ex.1120769 reads)

nElementsAfter

number of clonotypes in the dataset after all preprocessors have been applied (ex.30741 clones)

sumWeightBefore

total weight of all clonotypes after all preprocessors have been applied (ex. 125550). Note that all samples will have the same value for sumWeightBefore in this case, because downsampling was applied, thus all samples have been normalized to the same weight.

preprocessor#i

i-th part of the preprocessing chain (ex. preprocessor#1: Filter IGH chains)

nElementsBefore#i

number of clonotypes before i-th preprocessor has been applied (ex. nElementsBefore#1: 72121)

sumWeightBefore#i

total weight of all clonotypes before i-th preprocessor has been applied (ex. sumWeightBefore#1: 1120769)

nElementsAfter#i

number of clonotypes after i-th preprocessor has been applied (ex. nElementsAfter#1: 30741)

sumWeightAfter#i

total weight of all clonotypes after i-th preprocessor has been applied (ex. sumWeightAfter#1: 125550)

. . .

Various postanalysis tables contain information about each metric computed for each sample. For example, let's have a look inside pa.i.JUsage.IGH.tsv. This table contains frequencies for each J segment present in the sample :

sample	IGHJ3*00	IGHJ2*00	IGHJ1*00	IGHJ4*00
M1_4T1_Blood_S2.clns	0.246786	0.356726	0.12035	0.276137
M1_4T1_Blood_S6.clns	0.243162	0.376105	0.129136	0.251597
M1_4T1_BM_S4.clns	0.244986	0.288722	0.134162	0.332131
M1_4T1_BM_S8.clns	0.248443	0.286109	0.133891	0.331557
M1_4T1_DLN_S3.clns	0.247447	0.256957	0.0684667	0.427129

See full JUsage summary:

sample	IGHJ3*00	IGHJ2*00	IGHJ1*00	IGHJ4*00
M1_4T1_Blood_S2.clns	0.246786	0.356726	0.12035	0.276137
M1_4T1_Blood_S6.clns	0.243162	0.376105	0.129136	0.251597
M1_4T1_BM_S4.clns	0.244986	0.288722	0.134162	0.332131
M1_4T1_BM_S8.clns	0.248443	0.286109	0.133891	0.331557
M1_4T1_DLN_S3.clns	0.247447	0.256957	0.0684667	0.427129
M1_4T1_DLN_S7.clns	0.248857	0.258463	0.0734209	0.419259
M1_4T1_Tumor_S1.clns	0.160438	0.437953	0.0869295	0.314679
M1_4T1_Tumor_S5.clns	0.147081	0.467153	0.0821744	0.303592
M2_4T1_Blood_S2.clns	0.267965	0.307965	0.12372	0.30035
M2_4T1_Blood_S6.clns	0.27311	0.309741	0.125161	0.291987
M2_4T1_BM_S4.clns	0.280486	0.248323	0.108156	0.363035
M2_4T1_BM_S8.clns	0.284094	0.256734	0.102987	0.356185
M2_4T1_DLN_S3.clns	0.253222	0.264261	0.105448	0.377069
M2_4T1_DLN_S7.clns	0.256623	0.267256	0.104389	0.371732
M2_4T1_Tumor_S1.clns	0.302971	0.352816	0.107192	0.237021
M2_4T1_Tumor_S5.clns	0.277913	0.279315	0.122748	0.320024
M3_4T1_Blood_S10.clns	0.266125	0.28814	0.115484	0.330251
M3_4T1_Blood_S9.clns	0.266149	0.290179	0.117993	0.325679
M3_4T1_BM_S7.clns	0.25417	0.27687	0.163656	0.305305
M3_4T1_BM_S8.clns	0.253413	0.274401	0.164166	0.308021
M3_4T1_DLN_S11.clns	0.309574	0.227646	0.117523	0.345257
M3_4T1_DLN_S12.clns	0.31405	0.24184	0.114504	0.329606
M3_4T1_Tumor_S13.clns	0.158829	0.304691	0.223011	0.313469
M3_4T1_Tumor_S14.clns	0.156145	0.300573	0.226937	0.316344
M4_4T1_Blood_S2.clns	0.273971	0.313684	0.112911	0.299434
M4_4T1_Blood_S7.clns	0.269566	0.320542	0.11264	0.297252
M4_4T1_BM_S10.clns	0.371295	0.176581	0.115386	0.336738
M4_4T1_BM_S5.clns	0.359034	0.180552	0.111954	0.34846
M4_4T1_DLN_S4.clns	0.327861	0.270115	0.0523696	0.349654
M4_4T1_DLN_S9.clns	0.327949	0.27139	0.0540661	0.346595
M4_4T1_Tumor_S1.clns	0.266947	0.359864	0.121886	0.251304
M4_4T1_Tumor_S6.clns	0.256926	0.489064	0.15923	0.0947798
N1_control_Blood_S5.clns	0.273309	0.267073	0.171127	0.288491
N1_control_Blood_S6.clns	0.28047	0.267384	0.160573	0.291573
N1_control_BM_S1.clns	0.219785	0.313803	0.211661	0.254751
N1_control_BM_S2.clns	0.224994	0.319737	0.199012	0.256256
N1_control_LN_S3.clns	0.29499	0.252346	0.0789327	0.373732
N1_control_LN_S4.clns	0.300092	0.250834	0.081755	0.367319
N2_control_Blood_S5.clns	0.292911	0.263106	0.183855	0.260127
N2_control_Blood_S6.clns	0.290418	0.264644	0.182589	0.26235
N2_control_BM_S1.clns	0.253206	0.335986	0.106133	0.304675
N2_control_BM_S2.clns	0.254879	0.333023	0.106818	0.305281
N2_control_LN_S3.clns	0.302174	0.235643	0.0809558	0.381227
N2_control_LN_S4.clns	0.306045	0.243911	0.0812903	0.368753

Graphical output

MiXCR allows to export graphical results in PDF, EPS, PNG and SVG formats using exportPlots command.

For diversity metrics and CDR3 properties MiXCR allows to group data in different ways according to the submitted metadata.tsv and apply various statistical tests for group comparison.

Diversity

Let's reconstruct one of the figures from the paper. Let's say we want to look at the Normalized ShannonWiener diversity index. We will group samples by tissues and use separate facets for contol and experiment group. That can be easily done with a single command:

mixcr exportPlots diversity \
    --metadata metadata.tsv \
    --plot-type boxplot \
    --metric normalizedShannonWienerIndex \
    --primary-group tissue \
    --facet-by condition \
    --primary-group-values Tumor,LN,DLN,Blood,BM \
    pa/individual.json.gz \
    figs/diversity.svg

diversity.IGH.svg

Arguments explained:

--metadata: metadata.tsv is the name of metadata file.
--plot-type: boxplot is the type of plot to generate. See mixcr exportPlots for other options.
--metric:: normalizedShannonWienerIndex is the name of the metric to visualize. See diversity metrics for other options. If not specified multiple plots will be generated for each available diversity metric.
--primary-group: tissue is name of the column with metadata values from metadata.tsv to group samples by.
--primary-group-values: Tumor,LN,DLN,Blood,BM represents the order of --primary-group values on the plot (tissue in this case)
--facet-by: condition is name of the column with metadata values from metadata.tsv to group samples and represent on separate facets for each group.
postanalysis/individual.json.gz: the name of gzipped JSON file with all postanalysis results generated by `mixcr postanalysis individual
normalizedShannonWienerIndex.pdf: the name of output file. Also specifies the extension of the output file. One can use one of the following graphical out formats: .pdf, .eps, .png and .svg.

V usage

Now lets look at the J gene distribution among all samples.

mixcr exportPlots vUsage \
  --metadata metadata.tsv \
  --chains IGH \
  --palette sequential \
  --color-key tissue \
  pa/individual.json.gz \
  figs/vUsage.pdf

--palette: sequential. Determines color palette to be used for the heatmap. See gene segment usage plots for other options.
--color-key: tissue. Metadata column name.

vUsage.IGH.svg

Overlap postanalysis

Two samples overlap

Since our samples were prepared in replicas, it is often usefully to check if clone frequencies correlate between replicas. Let's take two replicas of one biological sample and overlap two repertoires. We will use mixcr overlapScatterPlot function:

mixcr overlapScatterPlot \
    --downsampling none \
    --chains IGH \
    results/M1_4T1_replica1_Blood.clns \
    results/M1_4T1_replica2_Blood.clns \
    figs/overlap.pdf

All-vs-All overlap

MiXCR also allows performing an overall overlap analysis using mixcr postanalysis overlap. But here, since there are a lot of samples we want to actually overlap groups of samples. Running the following command will perform pairwise overlap comparison between groups of samples with different tissue and condition values.

mixcr postanalysis overlap \
    --factor-by tissue,condition \
    --metadata metadata.tsv \
    --default-downsampling count-reads-auto \
    --default-weight-function read \
    --only-productive \
    --tables pa/postanalysis.overlap.tsv \
    --preproc-tables pa/preproc.overlap.tsv \
    results/*.clns \
    pa/overlap.tissue_condition.json.gz

--factor-by: tissue,condition defines the set of metadata columns for which a list of unique intersections will be created and used for group comparison

Other arguments are same as for individual postanalysis mentioned above.

This command will generate a set of files:

#Tsv files for every metric
postanalysis.overlap.F1Index.IGH.tsv
postanalysis.overlap.F2Index.IGH.tsv
postanalysis.overlap.JaccardIndex.IGH.tsv
postanalysis.overlap.PearsonAll.IGH.tsv
postanalysis.overlap.Pearson.IGH.tsv
postanalysis.overlap.RelativeDiversity.IGH.tsv
postanalysis.overlap.SharedClonotypes.IGH.tsv

# summary of applied preprocessors
preproc.overlap.IGH.tsv

# gzipped JSON with all results 
overlap.tissue_condition.json.gz

The tabular output for example for F2 metric will look like:

sample	Blood,4T1	BM,4T1	DLN,4T1	Tumor,4T1	Blood,control	BM,control	LN,control
Blood,4T1	1	0.123711	0.134151	0.357004	0.0477449	0.0576299	0.106747
BM,4T1	0.123711	1	0.0461159	0.115398	0.0728694	0.0295404	0.046996
DLN,4T1	0.134151	0.0461159	1	0.134484	0.0189902	0.0232703	0.0300021
Tumor,4T1	0.357004	0.115398	0.134484	1	0.0526398	0.0753527	0.128776
Blood,control	0.0477449	0.0728694	0.0189902	0.0526398	1	0.0592363	0.103798
BM,control	0.0576299	0.0295404	0.0232703	0.0753527	0.0592363	1	0.270242
LN,control	0.106747	0.046996	0.0300021	0.128776	0.103798	0.270242	1

Every overlap metric is also possible to present in a graphical format:

mixcr exportPlots overlap \
    --chains IGH \
    --palette density \
    --metric f2Index \
    pa/overlap.tissue_condition.json.gz \
    figs/overlap.tissue_condition.pdf

For list of available metrics see mixcr exportPlots overlap

overlap.tissue_condition.IGH.svg

For further details see overlap postanalysis reference.