mitool consensus
Assemble consensuses for sequences inside tag groups.(ex. UMI-based correction)
- Usage:
mitool consensus [OPTIONS] SOURCE DESTINATION
- Arguments:
SOURCE
- Input
*.mic
file DESTINATION
- Output
*.mic
file.
Options:
-g, --group-tag <TEXT>
- Tags to build groups of reads for consensus assembly (e.g.
CELL
,UMI
, etc...). If not set MiTool will use defaults if preset was specified on theparse
step. -a, --assemble-tag <TEXT>
- Tags to assemble consensuses for (e.g.
R1
,R2
,PAYLOAD
, etc...). If not set MiTool will use defaults if preset was specified on the parse step. --use-system-temp
- Use system temp folder for temporary files
- `-t, --threads
- Number of processing threads
-r, --report <PATH>
- Human readable report file. If file already exists, it will be overwritten.
-O <VALUE>
- Override consensus assembly parameters.
-h, --help
- Show this message and exit