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mixcr buildLibrary

Build custom V/D/J/C gene segment library. See how to create custom library.

Command line options

mixcr buildLibrary --species <species_name> --chain <chain> [--taxon-id <taxon_id>] (--v-genes-from-species <species> | [--v-genes-from-fasta <v.fasta> --v-gene-feature <gene_feature>]) (--j-genes-from-species <species> | [--j-genes-from-fasta <j.fasta> [--j-gene-feature <gene_feature>]]) [--d-genes-from-species <species> | [--d-genes-from-fasta <j.fasta> [--d-gene-feature <gene_feature>]]] [--c-genes-from-species <species> | [--c-genes-from-fasta <j.fasta> [--c-gene-feature <gene_feature>]]] [--do-not-infer-points] [--keep-intermediate] [--debug] [--force-overwrite] [--no-warnings] [--verbose] [--help] library.json[.gz]

--species <species_name>
Species name
--chain <chain>
Immunological chain
--v-genes-from-fasta <v.fasta>
FASTA file with Variable genes
--v-gene-feature <gene_feature>
Gene feature corresponding to Variable gene sequences in FASTA (e.g. VRegion or VGene)
--v-genes-from-species <species>
Species to take Variable genes from it (human, mmu, lamaGlama, alpaca, rat, spalax)
--j-genes-from-fasta <j.fasta>
FASTA file with Joining genes
--j-gene-feature <gene_feature>
Gene feature corresponding to Joining gene sequences in FASTA (JRegion by default)
--j-genes-from-species <species>
Species to take Joining genes from it (human, mmu, lamaGlama, alpaca, rat, spalax)
--d-genes-from-fasta <j.fasta>
FASTA file with Diversity genes
--d-gene-feature <gene_feature>
Gene feature corresponding to Diversity gene sequences in FASTA (DRegion by default)
--d-genes-from-species <species>
Species to take Diversity genes from it (human, mmu, lamaGlama, alpaca, rat, spalax)
--c-genes-from-fasta <j.fasta>
FASTA file with Constant genes
--c-gene-feature <gene_feature>
Gene feature corresponding to Constant gene sequences in FASTA (CExon1 by default)
--c-genes-from-species <species>
Species to take Constant genes from it (human, mmu, lamaGlama, alpaca, rat, spalax)
--taxon-id <taxon_id>
Taxon ID
--do-not-infer-points
Do not infer reference points
--keep-intermediate
Keep intermediate files
--debug
Print library debugging information
-f, --force-overwrite
Force overwrite of output file(s).
library.json[.gz]
Output library.